INTERACTION OF COPPER OXIDE NANOPARTICLES WITH BOVINE SERUM ALBUMIN BY SPECTROSCOPIC STUDIES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 5
Abstract
Objective: Since structural changes of adsorbed protein are necessary for cellular uptake of nanoparticles (NPs) it is of prime importance to know about structural changes of bovine serum albumin (BSA) when it interacts with CuO NPs–a potential new antitumor drug.Methods: CuO NPs prepared by sol-gel technique were characterized by x-ray diffraction (XRD) and tunneling electron microscope (TEM) techniques. The conformational changes induced by CuO NPs on BSA were studied by various spectroscopic techniques such as steady state and time-resolved fluorescence measurements. The changes in fluorescence emission parameters such as fluorescence intensity, fluorescence emission maximum and lifetimes of fluorescent residues in BSA were studied.Results: XRD analysis showed the average particle size as 32 nm. The TEM micrograph showed particles of different size varying from 10 to 45 nm. Fluorescence quenching was confirmed due to a decrease in fluorescence intensity of CuO NPs–BSA complex. The analysis of lifetime measurements indicated BSA contained two tryptophan (trp) residues that fluoresced in different environments. Static quenching mechanism was confirmed by time-resolved measurements when BSA interacted with CuO NPs.Conclusion: Minor structural changes of BSA protein were observed during the interaction studies.
Authors and Affiliations
Suja Abraham, Vellaichamy Parthasarathy
Keywords
Related Articles
- EP ID EP567495
- DOI 10.22159/ijpps.2018v10i5.24877
- Views 65
- Downloads 0
EFFECT OF ANGIOTENSIN CONVERTING ENZYME INHIBITORS AND ANGIOTENSIN-II RECEPTOR BLOCKERS ON PROTEINURIA OF HYPERTENSIVE PATIENTS IN STANDARD CARE PRACTICE
Objective: This study investigated the effect of angiotensin converting enzyme inhibitors (ACEIs) and angiotensin-ii receptor blockers (ARBs) on protein creatinine index (PCI) of patients with hypertension in a standard...
GERMINATION AND SEEDLING GROWTH OF SOME SELECTED AGRICULTURAL CROPS UNDER VARIOUS ABIOTIC STRESSES
Objective: The aim of this investigation was to evaluate the germination response of Vigna mungo, Vignaradiata, Zea mays and Brassica nigra to different stress conditions.  Methods: The germination responses of four ag...
A STUDY ON HYPERPHOSPHATEMIA AND EFFECT OF SEVELAMER ON CARDIAC ENZYME LEVELS IN CHRONIC KIDNEY DISEASE PATIENTS
Objective: Chronic Kidney Disease (CKD) is characterized by progressive loss of kidney function over a period of time. Sevelamer hydrochloride is a phosphate binding agent used to control serum phosphate levels in End St...
EFFECTIVENESS OF SPIRULINA MOUTHWASH ON REDUCTION OF DENTAL PLAQUE AND GINGIVITIS: A CLINICAL STUDY
Objective: The present study evaluated the effectiveness of Spirulina mouthwash on the reduction of dental plaque and gingivitis.Methods: A single-blind clinical trial was conducted among thirty patient’s aged 18-40 y...
RP-HPLC ASSAY METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF DILOXANIDE FUROATE AND ORNIDAZOLE IN TABLETS
Objective: To develop an accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for simultaneous quantitative estimation of Diloxanide furoate and Ornidazole in tablets and va...