Investigation of antiradical potential of different kinds of teas and extracts from these teas using antiradical activity units (TAU)
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2011, Vol 65, Issue 0
Abstract
Introduction: Green, black and pu-erh teas are known to have strong antiradical properties in comparison to other plant raw materials. In this study fifteen different teas belonging to three types, green, black, and pu-erh tea (five of each kind), were investigated for their antiradical properties. Antiradical activity of extracts and raw materials was measured using DPPH and ABTS•+ radicals. Material/Methods: The antiradical potential of teas was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS•+ (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt). The TAU515 and TAU734 (antiradical activity units) were defined with the tests of DPPH and ABTS•+, respectively, and the number of units was calculated per mg of extracts (TAU515/mg and TAU734/mg) and g of raw materials (TAU515/g and TAU734/g). Results/Discussion: When the extracts were investigated, the highest number of antiradical units (TAU515/mg; TAU734/mg) was found per mg of ethyl acetate extract obtained from green tea leaves assayed with DPPH and ABTS•+: 57.7±0.8; 106±2.0, units respectively. When the number of antiradical units TAU515/g was calculated per g of raw material, the highest antiradical potential (7601±92) was observed for green tea leaves. The greatest TAU734/g value (14 303±354) was also calculated for green tea leaves. The lowest value of antiradical activity units TAU515/g (684±30) and TAU734/g (1870±180) was calculated for pu-erh tea leaves. A high positive correlation was found between the antiradical activity of the raw materials and the content of phenolic compounds in these raw materials.
Authors and Affiliations
Damian Wojciechowski, Zbigniew Sroka, Andrzej Gamian
Keywords
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