Investigation of Gelatinase Gene Expression and Growth of Enterococcus faecalis Clinical Isolates in Biofilm Models
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2019, Vol 16, Issue 3
Abstract
Objectives: Enterococcus faecalis is the major reason for biofilm-related infections and it also interacts with Staphylococcus aureus in biofilms. Gelatinase (gelE) enzyme is an important virulence factor of E. faecalis for biofilm formation. This study aimed to compare the biofilm producing E. faecalis isolates from urine and urinary catheters. The influence of S. aureus on the growth of E. faecalis biofilm cells was also investigated in a dual biofilm model in vitro. Another aim was to evaluate E. faecalis gelE gene expression during biofilm formation. Materials and Methods: Firstly, crystal violet staining was used to measure the total biofilm biomass of the isolates. Secondly, plate counting was performed to determine the biofilm formation ability of E. faecalis isolates and the effect of S. aureus on E. faecalis biofilm formation. Finally, the gelE expression profile of the isolates was assessed by quantitative real time-polymerase chain reaction. Results: According to crystal violet staining and plate counting, all E. faecalis isolates were biofilm producers and the number of E. faecalis sessile cells increased in the presence of S. aureus. Among the 21 E. faecalis isolates, ten expressed high levels of the gelE gene, while eight of them had low expression profiles (p<0.05). Conclusion: When they grow together, S. aureus may give some advantages to E. faecalis such as increasing sessile cell growth. The expression of the gelE gene was not affected by E. faecalis biofilm formation of the isolates collected from the patients with urinary tract infections.
Authors and Affiliations
Didem KART, Ayşe Semra KUŞTİMUR
Keywords
Related Articles
- EP ID EP604428
- DOI 10.4274/tjps.galenos.2018.69783
- Views 146
- Downloads 0
The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines
Objectives: Natural products originating from plants have been used for many years in the treatment of various diseases, including cancer. Centaurea solstitialis subsp. solstitialis is used in Turkish folk medicine. This...
The Influence of Piperine on the Radioprotective Effect of Curcumin in Irradiated Human Lymphocytes
Objectives: Ionizing radiation (IR) induces DNA damage in normal cells, leading to genotoxicity. The radioprotective effects of co-treatment with curcumin and piperine were investigated against genotoxicity induced by IR...
Erratum
.
Opinions of Community Pharmacists About Collaboration with General Practitioners: A Descriptive Pilot Study After New Legislation in Turkey
Objectives: Committed legal regulations in primary healthcare services have resulted in certain issues and requirements about community pharmacy practices in Turkey. A professional collaboration between general practiti...
Development and Optimization of a Floating Multiparticulate Drug Delivery System for Norfloxacin
Objectives: Norfloxacin is a synthetic broad-spectrum antibacterial drug having poor bioavailability and pH-dependent solubility. The purpose of the present study was to develop a gastroretentive floating multiparticulat...