Investigation of subacute toxicity of antiparasitic preparation “Devimectin”
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue
Abstract
Introduction. In recent decades among the most widely used anti-parasitic preparations are ivermectin-based ones. In Ukraine, they are widely used in the treatment of cattle, sheep and goats against dictyocaulosis, stronhyloidosis, sarcoptoidias, subcutaneous, nasopharyngeal and gastric gadflys, etc. However, the preparations of this group are strong substances, although manufacturers indicate their low toxicity for warm-blooded animals. Limiting the data on the action of toxic doses of preparations of this group on the organism of mammals suggests the need for their comprehensive study. This will allow the development of preventive and therapeutic measures in the event of poisoning or manifestation of side effects. The goal of the work. To investigate the state of the leukocytes of blood of laboratory rats as an indicator of immunological reactivity of the organism under subacute toxicity of the antiparasitic preparation “Devimectin”. Materials and methods. The experiment used antiparasitic preparation “Devimectin” (active ingredient ivermectin), manufactured by LLC “EPIE” (Litin, Vinnitsa region, Ukraine). The experiment was conducted on non-breeding male rats weighing 260-290 g. Blood parameters were studied in 1, 7, 14, 30 and 40 days after acute intoxication with preparation “Devymectin”. A total leukocyte count (TLC) was calculated. The results were statistically processed. Results of research and discussion. It was found that in the period from the first to the seventh day after the intoxication of rats receiving a dose of 7 mg/kg, the white cell count decreased the content of segmental neutrophils, and in animals received a dose of 14 mg/kg, an increase in the content of these cells. The TLC in rats received 7 mg/kg dose of the preparation were correspondingly growing, and in animals with a dose of 14 mg/kg, it decreased. When analyzing the absolute content of different forms of leukocytes, absolute neutropenia was detected and decrease in the total amount of leukocytes of rats received 7 mg/kg of the preparation. In rats with a higher dose of poisoning – 14 mg/kg also recorded sharply expressed leukopenia. After the experiment, in the leucogram of the rats of both experimental groups, a significant reduction in the percentage of segmental neutrophils was recorded. In the cytoplasm of segmented neutrophils, toxic granularity and fragmentation of nuclei in experimental groups of animal were. The presence of such degenerative changes is confirmed by the high level of intoxication in experimental groups of rats. Conclusions and prospects for further research. The toxic doses of “Devymectin” in a day after preparation receiving leukopenia with changes in the content of neutrophils and lymphocytes developed in rats. In both experimental groups of animals, the percentage and absolute content of segmental neutrophils were reduced in 40 days after intoxication. The rate of lymphocytes increased, but their absolute amount remained below the control level, due to the preservation of leukopenia. Reducing the content of monocytes and eosinophils in the blood indicated a high level of intoxication in the rat’s body of both experimental groups. Further research will be aimed at studying the antiparasitic action of the preparation “Devicectin”.
Authors and Affiliations
Sergey Katyukha, Roman Sachuk, Sergii Zhyhaliuk, I. M. Lukyanik
Keywords
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- EP ID EP405629
- DOI 10.31073/vet_biotech32(2)-28
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