Development of methods for determining polycyclic aromatic hydrocarbons in water by gas chromatography-mass spectrometry after preliminary concentration
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2017, Vol 30, Issue
Abstract
Introduction. It is well-known that water is a prerequisite and an integral part of life on Earth. Therefore, the control of drinking water quality is very important for Ukraine especiallytaking into account a bad demographic situation in our country. Polycyclic aromatic hydrocarbons (PAHs) are dangerous carcinogens that penetrate into human body due to the consumption of drinking water. PAHs are dangerous to humans even at low concentrations due to bioaccumulation that leads to oncological diseases. Hence, the precise detection of PAHs is extremely important for humans safety. The goal of work was the development of the method for simultaneous determination of PAHs (indene(1,2,3-cd)pyrene, benzo(ghi)perylene, benzo(k)flyuorantenе, benzo(a)pyrene, benzo(b)flyuorantenе) by gas chromatography-mass spectrometry (GC-MS) and was the identification of analytes not only by retention time but also by using mass spectrum. Materials and methods. The drinking water was taken from dairy enterprises in Ukraine. The certified standard samples of indene(1,2,3-cd)pyrene, benzo(ghi)perylene, benzo(k)flyuorantenе, benzo(a)pyrene, benzo(b)flyuorantenе were used as referent materials. The solvent n-hexane and dodecane solution (Sigma-Aldrich, Germany) was utilized for samples preparation. Chromatography-mass spectrometer Thermo DSQ II equipped by chromatography column Agilent VF-5 was used for the development of detection method of PAHs. Results and discussions. The method of rapid simultaneous detection of 5 PAHs in water solution using gas chromatography-mass spectrometry is proposed. A desorption of polycyclic aromatic hydrocarbons was systematically studied. The identification of PAHs was performed by retention time and by supporting ions (m/z) comparing with the ratio of their intensity. The quantitative analysis was carried out by external standards for the peaks area. It was established that the quantitative desorption is carried out with water extractant by n-hexane in an amount of 25 cm3 at the temperature of 20 ± 1°C. It was found that the period of storage for PAHs standards at the temperature of + 4 ºC is 6 months and for interim solutions of PAHs standards is 3 months, respectively. Conclusions and prospects for further research. The advantage of the proposed method is high metrological characteristics and reproducibility of results. The limit of quantification of (LOQ) for indene(1,2,3-cd)pyrene, benzo(ghi)perylene, benzo(k)flyuorantenе, benzo(a)pyrene, benzo(b)flyuorantenе was reached of 1х10-2 µkg/liter. Our proposed method was successfully tested in the laboratory studies at enforcement of EC 98/83 directives. The method was accredited by National Accreditation Agency of Ukraine.
Authors and Affiliations
Tatyana Yevtushenko, Julia Omelchun
Keywords
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- EP ID EP267281
- DOI 10.31073/vet_biotech30-08
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