Investigation on Biodecolorization and Molecular Fractionation of Melanoidin Pigment using Cladosporium cladosporioides
Journal Title: International Journal of Research in Chemistry and Environment (IJRCE) - Year 2012, Vol 2, Issue 4
Abstract
This study presents the utilization of Cladosporium cladosporioides as potential fungal strain for the decolorization and molecular fractionation of synthetic melanoidin pigment which is a recalcitrant biopolymer. Single factorial experiment was conducted for determining the optimum process parameters by varying concentration of carbon, nitrogen source and concentration of the pigment for achieving maximum decolorization. During the experiment a maximum decolorization of 58.5% was obtained on 6th day with when incubated with an optimum condition of 0.5% (w/v) fructose, 0.25 %(w/v) peptone and 0.5%(w/v) pigment concentration at pH 5 and 30o C. Decolorization and degradation of the pigment was confirmed by Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) method, Gel filtration chromatography and UV-Spectrophotometer analysis. This investigation could be an approach towards control of environmental pollution & health hazards of rural people
Authors and Affiliations
Ravikumar R. , Saravanan K.
Keywords
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