INVESTIGATIONS OF THE LEVEL OF OLIGONUCLEOTIDE IMMOBILIZATION ON THE SURFACE OF GOLD NANOPARTICLES USING EXPERIMENTAL APPROACH WITH FLUOROPHORE CY3
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2014, Vol 11, Issue 3
Abstract
The level of oligonucleotide immobilization on the surface of gold nanoparticles for the nanobiosensor development was studied using a proposed experimental approach. This approach for evaluation of the amount of immobilized material is based on the measurement of the residual concentration of free (unimmobilized) thiolated oligonucleotides with covalently attached fluorophore Cy3. It was shown that the efficiency of immobilization of oligonucleotides on the surface of citratestabilized gold nanoparticles is strongly dependent on the ionic strength of the medium as well as on the temperature and time of immobilization. Along with increase of ionic strength of the medium, two processes are observed: change in ionic strength (from 0 to 15-20 mM) leads to more effective immobilization of oligonucleotides, and a further increase of ionic strength leads to aggregation of gold nanoparticles, which impedes further effective immobilization of oligonucleotides.
Authors and Affiliations
М. Matsishin, A. Rachkov
Keywords
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- EP ID EP399698
- DOI 10.18524/1815-7459.2014.3.108236
- Views 76
- Downloads 0
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