Isolation and antibiotic susceptibility of Escherichia coli from urine sample received in SMS Medical College, Jaipur
Journal Title: International Journal of Medical and Health Research - Year 2019, Vol 5, Issue 4
Abstract
Background: Escherichia coli is the most clinically relevant and multiple drug resistant bacterial pathogen causing urinary tract infections (UTIs). In the community, women are more prone to develop UTI. About 20% of women experience a single episode of UTI during their lifetime, and 3% of women have more than one episode of UTI per year. In males, with advancing age, the incidence of UTI increases due to prostate enlargement and neurogenic bladder. Objective: To assess the changing susceptibility pattern of Escherichia coli to antimicrobial agents in urine samples. Material and Method: A total of 160 urine samples collected from patients attending hospital both OPD and IPD. A calibrated loop method (semi quantitative method) was used for the isolation of bacterial pathogens from urinary samples. Significant isolates were identified by conventional methods according to the standard laboratory protocol, including colony morphology, gram staining and biochemical reactions. The antibiotic sensitivity test was performed by modified Kirby Bauer disc diffusion technique. Result: Among 625 clinically suspected cases of urinary tract infection 250 (40%) were significant growth and 375 (60%) were sterile. Out of 250 positive culture Escherichia coli was predominant 160 (64%) among that 92(57%) were ESBL producer while 68(43%) were Non ESBL producer. Escherichia coli showed highest sensitivity to Polymixin (100%) followed by Tigicycline (95%). Conclusion: A high isolation rate of Escherichia coli from urine samples of clinically-suspected UTI shows a good correlation between clinical findings. Our study highlights the need for the development of protocol for rational use of antibiotics.
Authors and Affiliations
Mahesh Kumar Yadav, Rakesh Kumar Maheshwari, Ramesh Kumar Mishra, Bharti Malhotra
Keywords
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