Isolation and Characterization of Phosphate Solubilising Bacteria from a Discrete Ecological Niche in Navi-Mumbai, Maharashtra, India
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2016, Vol 4, Issue 11
Abstract
Phosphorus usually occurs in insoluble form, but is an important element for plant growth. Some microbes can convert insoluble phosphate to soluble form making them available for plants. An effort on isolation and characterization of phosphate solubilizing bacteria from two different mangrove inhabitat sampling sites was attempted in this study. 30 phosphate solubilizing bacteria were isolated on Pikovaskaya’s agar. Eight of these isolates showed sharp halos on Pikovaskaya’s agar assay plate supplemented with 0.003% Rose Bengal. Maximum phosphate solubilisation in broth was shown by isolate S4RWB. Decline in pH value of media was observed from 7.2-2.5 within 48h for some of the isolate. The efficiency of Phosphate solubilisation of these isolates were also characterised in presence of various carbon and nitrogen sources and the effect of pH and temperature was also evaluated.
Authors and Affiliations
S. Harpude, J. Patki, S. Singh
Keywords
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