Isolation and characterization of thermostable lipases from themophilic bacteria isolated from hot water spring
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2018, Vol 26, Issue 2
Abstract
Twelve isolates were screened for lipases production from hot water spring, Vajreshwari, Thane, Maharashtra on a Nutrient agar and nutrient broth medium consisting of olive oil. The isolate showed highest lipase activity was VIII which later was identified as phingomonas paucimobilis. The effect of pH, temperature and substrate concentration was studied on partially purified lipases by ammonium sulphate precipitation from Sphingomonas aucimobilis. The partially purified lipase had maximal activity at pH of 7.The optimum temperature for the hydrolysis of olive oil at 60°C.The lipases has ability to hydrolyse used fried oil. The transesterification activity of lipases was used to synthesis biodiesel. The analysis of synthesized biodiesel was performed by FTIR( Fourier transform infrared spectroscopy) and free fatty acid content.
Authors and Affiliations
R J Pungle, S G Sapkal, B D Vyawahare, P R Bhandwalkar, S B Bedwal
Keywords
Related Articles
- EP ID EP365971
- DOI -
- Views 90
- Downloads 0
Unusual Site for Branchial Cyst Occurrence
Branchial cleft cysts typically present as a unilateral, fluctuant soft tissue swelling that is localized deep to the anterior border of sternocleidomastoid in the lateral aspect of the neck. They are often discovered la...
Eradicating bacteria with silica and polyethylene glycolcoated gold nanoparticles using photothermal therapy
Various species of bacteria have adapted to manmade chemicals and antibiotics, developing into superbugs posing a threat to humankind, and soon creating a future where antibiotics are futile against various diseases. In...
Significance of glycemic status in patients of major depressive disorder with suicidal behaviour
Background: Suicidal ideation is common in major depressive disorder, and it is thought to be a strong contributing factor for completed or attempted suicide. Hyperglycemia and hyperinsulinemia may increase the risk of d...
Predisposing Risk Factors for Neonatal Seizures in Low Birth Weight Babies: Case Control Study
Objective: To study the predisposing risk factors for neonatal seizures in low birth weight babies. Design: Prospective case control study. Settings: Neonatology Unit at tertiary care hospital. Outcome measures: Associat...
Prevalence and severity of restless leg syndrome in pregnant women of Latur district
Background: Restless legs syndrome (RLS), a common sensorimotor disorder, has a wide range of severity from merely annoying to affecting sleep and quality of life severely enough to warrant medical treatment. Previous ep...