Isolation and characterization of thermostable lipases from themophilic bacteria isolated from hot water spring
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2018, Vol 26, Issue 2
Abstract
Twelve isolates were screened for lipases production from hot water spring, Vajreshwari, Thane, Maharashtra on a Nutrient agar and nutrient broth medium consisting of olive oil. The isolate showed highest lipase activity was VIII which later was identified as phingomonas paucimobilis. The effect of pH, temperature and substrate concentration was studied on partially purified lipases by ammonium sulphate precipitation from Sphingomonas aucimobilis. The partially purified lipase had maximal activity at pH of 7.The optimum temperature for the hydrolysis of olive oil at 60°C.The lipases has ability to hydrolyse used fried oil. The transesterification activity of lipases was used to synthesis biodiesel. The analysis of synthesized biodiesel was performed by FTIR( Fourier transform infrared spectroscopy) and free fatty acid content.
Authors and Affiliations
R J Pungle, S G Sapkal, B D Vyawahare, P R Bhandwalkar, S B Bedwal
Keywords
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