Isolation and Detection of Marine Microorganisms and Evaluation of In-Vitro Insecticidal Activity of Ethanolic Crude Extracts of Marine Streptomyces sp. Against Larvae of Sitophilus oryzae
Journal Title: Scholars Academic Journal of Pharmacy - Year 2013, Vol 2, Issue 4
Abstract
Marine microorganisms, whose immense genetic and biochemical diversity is only beginning to be appreciated, look likely to become a rich source of novel chemical entities for the discovery of more effective drugs. The present study was performed to evaluate the insecticidal potential of Actinomycetes isolated from the soils of the Dublarchar and Kochikhali, Bangladesh. The actinomycete isolates were identified by various parameters such as colony morphology, spore arrangement, staining, and biochemical reactions. Insecticidal activity of different concentrations of ethanolic extract of the isolates was determined against the second instar larvae of Sitophilus oryzae. The larvicidal effect, in terms of mortality of larvae, of the extracts was determined by counting the number of dead larvae after 24 hours. Ten actinomycete isolates were recovered from the soil sample and one was subjected to identify (Due to potent antibacterial activity) as the species of Streptomyces on the basis of microscopic, biochemical, and staining characteristics. The insecticidal potential of ethanolic extracts, in terms of larval mortality, was found to be dose dependent. The isolate showed a marked insecticidal activity. At a concentration of 24mg/ml, the isolate caused 100% mortality of the larvae. The lethal conc. 50 was found 3.16mg/ml. Isolation and characterization of active constituents from the ethanol extract possessing insecticidal potential are to be investigated. Keywords: Streptomyces, Antibiotics and Insecticides, Sitophilus oryzae,
Authors and Affiliations
Md. Anwarul Haque, Rishikesh . , Mohammad Sayful Islam, Md. Ajijur Rahman
Keywords
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