ISOLATION AND HPTLC ESTIMATION OF AESCULIN FROM OXYSTELMA ESCULENTUM
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 6
Abstract
Oxystelma esculentum R. Br. (Family: Asclepiadaceae) is a perennial twiner growing in water-logged areas of the Indian sub-continent. It is used traditionally as diuretic, laxative and anti-ulcer. The present work deals with the isolation and structure elucidation of Aesculin from O. esculentum and HPTLC method development for its estimation. The methanolic extract of the entire plant of O. esculentum was subjected to further fractionation followed by pilot TLC experiments and column chromatography. This yielded a pure, white, crystalline solid which resolved at Rf 0.50 upon TLC of methanolic extract using the mobile phase ethyl acetate: methanol (6:1). This compound was subjected to UV, IR, LC-MS and 1H-NMR spectral analysis and its structure elucidation revealed it to be Aesculin. A novel HPTLC method for the estimation of Aesculin from O. esculentum was developed, which was found to be 0.6410.15%w/w. The method developed was found to be easy, simple, precise, efficient, accurate, reproducible, specific and sensitive, and could serve as a suitable tool for routine analysis and phytochemical authentication of O. esculentum.
Authors and Affiliations
Devang Pandya , Indermeet Anand
Keywords
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