Isolation and Identification of Fungi Causing Dermatophytosis In and Around Patna, Bihar
Journal Title: International Journal of Medical Research Professionals - Year 2018, Vol 4, Issue 6
Abstract
Introduction: Fungus are eukaryotic Protista. Fungal infections are classified into superficial mycosis, subcutaneous mycosis and systemic mycosis according to the tissues involved. Superficial mycosis are prevalent in most parts of the world. The dermatophytes are closely related keratinophilic fungi causing Tinea infection, by virtue of their ability to degrade keratin and thus to invade the skin, hair and nails. Tinea infection presents as an annular lesion with advancing, inflamed and raised border. Hot and humid climate in the tropical and subtropical countries like India makes dermatophytosis very common superficial fungal skin infection. Inadvertent application of broad-spectrum antibiotics has raised prevalence of dermatophytosis. So, isolation and identification of fungi is essential for appropriate treatment and for epidemiological purposes. Materials and Methods: 100 patients having a skin, hair and nail infection were selected. Skin scales, crusts and pieces of nails were collected by scrapping across the inflamed margin of the lesion. Hairs were epilated with the sterilised forceps. Samples were examined in microbiology department. All samples were examined for fungal infection by means of microscopy, slide culture, urease test and hair perforation test. Species were identified on the basis of their growth characteristic and microscopic morphology. Results: Out of 100 clinically diagnosed cases of Tinea 78 cases were positive by culture and microscopy and 22 cases were negative for fungal infection. Tinea corporis (45%) was the most common clinical type followed by T. cruris (19%), T. unguium (16%) followed by T. capitis (8%) then T. corporis with cruris (6%) then T. pedis (4%). Among Tinea corporis, T. rubrum (50%) was the most common dermatophyte isolated followed by T.mentagrophyte (33.33%). Among Tinea cruris, T.rubrum (60%) was the most common dermatophyte isolated followed by T.mentagrophyte (30%). T. mannum was the least common clinical type found with 4 cases. Conclusion: 70 cases were culture +ve out of which 10 were KOH -ve. 22 cases were both culture -ve and KOH -ve. However 8 cases were culture -ve but KOH +ve. In our study T.rubrum was the most common (48.57%) followed by T.mentagrophyte (30%).
Authors and Affiliations
Babita Kumari, Shweta Singh, Pratulya Nandan, R K Srivastava
Keywords
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- EP ID EP533974
- DOI 10.21276/ijmrp.2018.4.6.048
- Views 78
- Downloads 0
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