ISOLATION AND IDENTIFICATION OF HELICOBACTER PYLORI FROM CULTIVATED AND COMMERCIALLY SOLD VEGETABLES AND SALAD FROM SOME SELECTED TOWNS (NNEWI, AND ODEKPE) IN ANAMBRA STATE
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2017, Vol 3, Issue 7
Abstract
Helicobacter pylori plays an important role in peptic ulcer disease and gastric carcinoma.This study was conducted to isolate and identify Helicobacter pylori from 64 cultivated and 60 commercially sold vegetables and salad materials in Odekpe and Nnewi towns in Anambra state Nigeria using conventional culture technique on selective Columbia blood agar base and antibiotic supplement(Oxoid, England). Identification was done using biochemical tests. Antibiotic susceptibility was also done with Kirby Bauer disc-diffusion method using Mueller Hinton agar (HiMedia Laboratories, Mumbai, India) according to the Clinical Laboratory Standards Institute.(CLSI 2012).Results revealed that a total of 6(9.4%) and2(3.3%) was isolated from vegetables from Odekpe and Salad materials from Nnewi towns with no significant difference (P>0.05;P0.171,X21.873). Pumpkin leaves was more contaminated in Odekpe 1(16.7%) and leek in Nnewi.2(100%).Unwashed samples recorded higher prevalence than washed, with no significant difference. (P>0.05;p0.144,x 2.138). Animals walking around farm, type of animal and source of water for irrigation showed marked significant association with the presence of organism. (P<0.05; P0.007, 0.009, 0.002: X27.30, 6.84, 15.28).Highest resistance to antibiotics was recorded in Nalidixic acid 8(100%) and Cephalothin 8(100%) and susceptibility to Chloramphenicol 8(100%) and Ciprofloxacin 8(100%). In conclusion, vegetables from Odekpe town were highly contaminated with H. pylori. Unwashed vegetables pose a threat to H. pylori contamination. Knowledge on type of animal walking around the farm, water source used for irrigation should be disseminated as a risk factor to H. pylori spread.
Authors and Affiliations
Obeagu E. I.
Keywords
Related Articles
- EP ID EP649505
- DOI -
- Views 115
- Downloads 0
RESPONSE OF THE GROWTH AND PRODUCTION OF MELON (CUCUMIS MELO L.) ON PLANT HORMONE AND COW MANURE APPLICATION
Melons are one of the main priority crops that receive attention among other horticultural crops, as the price of melons is relatively higher. This study aimed to produce higher quality melon fruit with larger diameter a...
SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF SOME 2-NAPHTHYLACETATE MANNICH BASES
Using 2-naphthylacetate as active hydrogen component, the following Mannich bases were synthesized via a general synthesis protocol: 1-hydroxymethyl-1-piperidinomethyl-2-naphthylacetate(I); 1,1-dimorpholinomethyl-2- Naph...
CLINICAL PHARMACOKINETICS CONCEPTS IN THE DRUG DISCOVERY AND DEVELOPMENT PROCESS OF PHYTOMEDICINES IN SOME DEVELOPING COUNTRIES
Clinical Pharmacokinetics can be defined as the study of the relationship between drug dosage regimens and the concentration time profiles. There has been in the last decades a significant progress in the field of Clinic...
THE EFFECT OF JALA NETI IN THE MANAGEMENT OF VATAJA PRATISHYAYA W.S.R. TO ALLERGIC RHINITIS – A CASE STUDY
Allergic Rhinitis is one of the commonest diseases mostly occurring during change of season or exposure to dust, cold, pollens etc.[1] It is highly prevalent and it has been recorded as, 20-30% of people are suffering fr...
A REVIEW ON THE MICROBIAL LIPASES FOR ADVANCED INDUSTRIAL APPLICATIONS
The lipids hydrolysis is an enzyme catalyzed reaction which involves the formation of low molecular weight compounds. In processing Industry of several products, lipase is among one of the best commercial enzyme. For Lip...