Isolation and Identification of Microorganism to Study of Their Potential to Degrade Harmful Azo Dyes by the Enzyme Azoreductase Produced by the Microorganism
Journal Title: Scholars Academic Journal of Biosciences - Year 2015, Vol 3, Issue 6
Abstract
Abstract: Azo Dyes are used commonly in different industries. Through the biotransformation of dye compounds, various dye precursor and carcinogenic aromatic amines are generated which leads water pollution and affect phytoplankton’s, water animals and human beings. Thus there is an aurgent need to remove these dyes from various industrial effluents. Enzymatic degradation of dyes through microorganisms was an effective and eco friendly process. In this study different microbes that produce dye degrading enzymes were isolated and identified as B. Brevis, B. Pantothenticus (Bacteria) and A. Niger, Fusarium (Fungus). B. Brevis and B. Pantothenticus showed degradation for methyl red at gm/l dye concentration and for congo red (when the concentration was decreased). Percentage dye degradation of B. Pantothenticus was 91.66 (For methyl red) and 75.37 (For congo red). Percentage dye degradation through A. Niger was 85.71 (For methyl red).The enzyme responsible for dye reduction was purified by Ion exchange chromatography. Enzyme assay was performed for the purified enzyme, it is found that the optimum pH and temperature was 7 and 40ºC respectively. Purity of enzyme was determined by SDS-PAGE and the m.wt. of enzyme was64 KD. Keywords: Azo Dyes, Congo red, Methyl red.
Authors and Affiliations
Huma Naz Siddiqui, Dr Arunima Karkun, Simran Jangir
Keywords
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