Isolation and Identification of Nah-B Gene from Bacteria Isolated from Effluents & Testing its Hydrocarbon Degrading Efficiency
Journal Title: International journal of Emerging Trends in Science and Technology - Year 2014, Vol 1, Issue 9
Abstract
One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Accidental releases of petroleum products are of particular concern in the environment. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxin organic pollutants. Bioremediation is the promising technology for the treatment of these contaminated sites since it is cost-effective and will lead to complete mineralization. In this project hydrocarbon degrading bacteria isolated from hydrocarbon contaminated soil by using nutrient agar medium and mineral salt agar medium supplemented with liquid petrol and diesel which was used as the sole carbon source for bacterial growth was performed. The bacteria was identified by using Bergey’s manual of bacterial classification. The nah gene which responsible for the hydrocarbon degradation was amplified. The sequence has similarity about 90% to nahB gene.
Authors and Affiliations
Shyam Prasad. V
Keywords
Related Articles
- EP ID EP221127
- DOI -
- Views 86
- Downloads 0
Secure Cloud Based Smart Grid Application
The present system of energy billing is error prone and also time and labor consuming. Errors get introduced at every stage of energy billing like errors with electro-mechanical meters. In the Modern world intelligent co...
Design and Development of Hyper Spectral Image Classification Using Enhanced Mean Shift Segmentation in Image Mining
Hyper spectral imaging is becoming an important analytical tool for generating land-use map. High dimensionality in hyper spectral remote sensing data can guaranty in principle a detailed discrimination of the observed s...
HASH Mechanism to Secure the AODV Protocol in MANETs
Mobile ad-hoc networks are self forming network without any fixed infrastructure. An ad hoc network does not have fixed infrastructure, it means a network without the standard routing infrastructure like fixed routers an...
Universal Data Management through Web Base Configurable System
This paper presents universal data management through web base configurable system. Universal data stands for most of analog signal which are useful for mankind and industrial system. 24bit ADC provides high resolution d...
Software Protection against Piracy and Reverse Engineering using Software Watermarking Technique
The rise in use of Internet and byte code languages such as Java byte code and Microsoft’s Common Intermediate language have made copying, decompiling and disassembling software easier with the rapid development of Inter...