Isolation and Molecular Identification of Endophytic bacteria from Red Betel Root (Piper crocatum Ruiz & Pav) as a Producer of Anti-Bacterial Compounds
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 1
Abstract
This research conducted to find out the capabilities of endophytic bacteria isolate from root of red betel plants (Piper crocatum Ruiz & Pav) in inhibiting the growth of bacteria Streptococcus aureus, escherichia coli, Klebsiella pneumoniae, Streptococcus mutans, and to identify the endophytic potensial bacteria. this research was devide into 3 stages, endophytic bacteria isolation, antibacterial test, biochemical test and molecular identification upon the potensial endophytic bacteria. It was found 2 isolates from root of the red betel plants; only One (1) isolat can be inhibition S. aureus and E. coli. Based on 16S rRNA molecular identification, isolates of endophytic bacterial of red betel root which have inhibitory effect are closely related to Bacillus pumilus strain AUEC29.
Authors and Affiliations
Lalu Wira Zain Amrullah, Dwi Soelistya Dyah Jekti, Lalu Zulkifli
Keywords
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- EP ID EP414996
- DOI 10.9790/264X-04012732.
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