Isolation and Molecular Identification of Endophytic bacteria from Red Betel Root (Piper crocatum Ruiz & Pav) as a Producer of Anti-Bacterial Compounds
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 1
Abstract
This research conducted to find out the capabilities of endophytic bacteria isolate from root of red betel plants (Piper crocatum Ruiz & Pav) in inhibiting the growth of bacteria Streptococcus aureus, escherichia coli, Klebsiella pneumoniae, Streptococcus mutans, and to identify the endophytic potensial bacteria. this research was devide into 3 stages, endophytic bacteria isolation, antibacterial test, biochemical test and molecular identification upon the potensial endophytic bacteria. It was found 2 isolates from root of the red betel plants; only One (1) isolat can be inhibition S. aureus and E. coli. Based on 16S rRNA molecular identification, isolates of endophytic bacterial of red betel root which have inhibitory effect are closely related to Bacillus pumilus strain AUEC29.
Authors and Affiliations
Lalu Wira Zain Amrullah, Dwi Soelistya Dyah Jekti, Lalu Zulkifli
Keywords
Related Articles
- EP ID EP414996
- DOI 10.9790/264X-04012732.
- Views 140
- Downloads 0
Degradation of Cellulosic Substrates By Bacteria Isolated From Earthworm
A study had been carried out to evaluating degradation ability of cellulosic substrates by bacteria isolated from earthworm. Bacteria were isolated by hungate selective media with Carboxy Methyl Cellulose/CMC as selectiv...
Biochemical, Sensorial And Novel Product Development From Carica Papaya Seeds: An Insight
Carica papaya, commonly known as papaya, pawpaw and papita; is a delicious fruit with loaded nutrients having beneficial effect on human body. Papaya also has antioxidant effects and anticancer properties which improve h...
Molecular Characterization and Alchohol Tolerance of Saccharomyces Cerevisiae Used In Bioethanol Production From Waste Paper
Sacchaomyces cerevisiea has been widely used in the production of bioethanol from several substrates. This study portrays the molecular characterization and alcohol tolerance of Sacchaomyces cerevisiea used in the produc...
Antibiofilm effects of Adhatoda Vasica Nees. extracts in comparison with commercial vasicine on Streptococcus Pneumoniae
Medicinal plants like Adhatoda vasica Nees. are used traditionally in Tripura to cure pneumonia, cough and cold. Pneumonia is a lung infection with cough, fever and hard time breathing. Pneumonia is mainly caused by bact...
Antimicrobial Activity Of Murraya Koenigii And Zingiber Officinale Plants On Drug Resistant Pathogens Isolated From Clinical Specimens And Preliminary Phytochemical Analysis Of The Crude Extract Preparations.
The aim of the present study was to investigate the antimicrobial activity and phytochemical screening of Murraya koenigii and Zingiber officinale plant extracts. Ethanol and Methanol were used as the solvents for extrac...