Effect of Ethanol Extract of Moringa Oleifera Leaves in Protecting Anaemia Induced in Rats by Aluminium Chloride

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Effect of Ethanol Extract of Moringa Oleifera Leaves in Protecting Anaemia Induced in Rats by Aluminium Chloride

Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 6

Abstract

Moringa oleifera leaf is a nutritious vegetable with variety of the rapeuticuses. This study was carried out to determine the effectiveness of ethanol leaf extract of Moringa oleifera in the treatment of anaemia induced in albino rats by administration of AlCl3. Phytochemical screening was carried out on M.oleifera leaf extract according to standard procedure. Fifty albino rats were divided into six groups each consisting of eight animals. The control group (group1) received normals a line solution only, group2 was the negative control group and was administered 0.5mg/kg of AlCl3. Group3, 4 and 5 were the treatment groups and in addition to 0.5mg/kg body weight of AlCl3 received M.oleifera extract of 500, 1000 and 2000 mg/kg respectively. Group 6 was the extract control group and were given 500 mg/kg body weight of M.oleifera. The phytochemical analysis of the ethanol extract of M.oleifera leaf showed the presence of tannins, saponins, glycosides, terpenoids, steroids, flavonoids and alkaloids. The result showed that AlCl3led to significant decrease (P<0.05)inred blood cell count (RBC), hemoglobin (Hb), and hematocrit (PCV) compared with the positive control rats (not administered AlCl3). The result also showed that administration of M.oleifera leaf extract at doses of 500, 1000 and 2000 mg/kg caused significant dosed ependent decrease in serum AST and ALT activities compared with then egative control group (administered AlCl3 without treatment). However, no significant difference (P>0.05) was observed in serum AST and ALT activities of the extract control group (administered 500 mg/kg of extract without AlCl3) compared with the normal control group while administration of M.oleifera leaf extract at doses of 500, 1000 and 2000 mg/kg caused significant dose dependent increase in serum total proteins. No significant difference (P>0.05) was observed in serum total proteins of anaemic rat administered M.oleifera extract at doses of 500 mg/kg compared with the negative control group (AlCl3). The normal control group and the extract group had progressive increase in body weight throughout the study period (28days) while the group of rats given AlCl3 with M.oleifera extract at doses of 500, 1000 and 2000 mg/kg showed aninitial decrease in body weight for 2 weeks of treatments and a significant improvement in body weight after the third week. This study has shown that M.oleifera leaf extract has the potential to ameliorate the damaged blood parameters in AlCl3 induced anaemia in rats.

Authors and Affiliations

Ameh S. S. , Alafi O. F.

Keywords

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EP ID EP430891
DOI 10.9790/264X-0406013452.
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