Isolation and Purification of antibacterial proteins from hemolymph of Marine gastropods dwelling at rocky shores of Kanyakumari, Southeast coast of India
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 2
Abstract
Proteins from body fluid (hemolymph) of two marine gastropods Nerita albicilla and Purpura bufo collected from the coastal regions of Kanyakumari were precipitated with ammonium sulphate and purified using ion-exchange chromatography. The fractions obtained were tested for their antibacterial potential against human pathogens. Fractions F2 and F4 from N.albicilla showed higher inhibitory activity against Salmonella typhi, Streptococcus mutans and Staphylococcus aureus. The molecular weight of this antibacterial protein present in the fractions was determined with the help of SDS PAGE and it was found to be 30KDa. Further, characterization of this active protein paves the way for the development of new antibiotic against harmful human pathogens in future.
Authors and Affiliations
Sharmin Vini S, Viju N, Ezhil Raj N, Mary Josephine Punitha S
Keywords
Related Articles
- EP ID EP546111
- DOI -
- Views 46
- Downloads 0
SEM study of some allied species of Cyperus L. from Khandesh region of Maharashtra, India
The nut morphology of 08 allied species of Cyperus L. from Khandesh region of Maharashtra was examined under LM and SEM. The nuts exomorphic characters that is diagnostic at species level are, nut shape, size, dimensions...
Diversity, Distribution and Status of the Amphibian fauna of Sangli district, Maharashtra, India
30 species of amphibians were reported during a survey belonging to 19 genera of 9 families and 2 orders from Sangli district, Maharashtra, India, during June 2013 to May 2017. Out of 30 species recorded, 19 species are...
Encapsulation of Lactic acid bacteria in calcium alginate beads for higher bacteriocin production
Lactic acid bacteria (LAB) strains LAB -A, LAB -B and LAB-C isolated from batter of idli, very popular fermented food of south India and identified to species level using 16 S rRNA sequencing as Pediococcus acidilactici...
Efficacy study of some antiseptics and disinfectants
Antiseptics and Disinfectants are widely used in hospitals and other health care centers to control the growth of microbes on both living tissues and inanimate objects. Different pathogens responded different antiseptics...
Comparative foliar micro morphological studies on some Tephrosia Pers. species of Indian Thar Desert
A comparative foliar micro morphological study of four species of Tephrosia Pers. viz. Tephrosia purpurea, Tephrosia uniflora subsp. petrosa, Tephrosia villosa, Tephrosia wallichii of Indian Thar desert was carried out t...