Isolation and Purification of antibacterial proteins from hemolymph of Marine gastropods dwelling at rocky shores of Kanyakumari, Southeast coast of India
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 2
Abstract
Proteins from body fluid (hemolymph) of two marine gastropods Nerita albicilla and Purpura bufo collected from the coastal regions of Kanyakumari were precipitated with ammonium sulphate and purified using ion-exchange chromatography. The fractions obtained were tested for their antibacterial potential against human pathogens. Fractions F2 and F4 from N.albicilla showed higher inhibitory activity against Salmonella typhi, Streptococcus mutans and Staphylococcus aureus. The molecular weight of this antibacterial protein present in the fractions was determined with the help of SDS PAGE and it was found to be 30KDa. Further, characterization of this active protein paves the way for the development of new antibiotic against harmful human pathogens in future.
Authors and Affiliations
Sharmin Vini S, Viju N, Ezhil Raj N, Mary Josephine Punitha S
Keywords
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