ISOLATION AND PURIFICATION OF CHITINOLYTIC ENZYMES OF RUMEN CILIATES Eudiplodinium maggii
Journal Title: Progress on Chemistry and Application of Chitin and its Derivatives - Year 2010, Vol 0, Issue
Abstract
Results of the earlier studies suggested an involvement of ciliates Eudiplodinium maggii in the digestion and metabolism of chitin in the rumen. In the presented paper we described the results on the preliminary identification and characterization of chitinolytic enzymes of this ciliate as well as the method of their purification. The protozoal crude enzyme preparation was used as source of enzymes, whereas the molecular filtration on Sephadex G-150 (single step method) or separation of protein by precipitation with ammonium sulfate followed by molecular filtration (two step method) were applied to purify the identified enzymes. The identification studies resulted in the detection of endochitinase, exochitinase and N-acetyl-D-glucosaminidase. The highest activity of identified enzymes were obtained in 4.0 - 4.5 pH and at 45 - 50 °C. Results of the comparative study on purification procedures showed that the single-step method enabled us to obtain enzymes of higher purity and higher activity than the two-step purification method.
Authors and Affiliations
Renata Miltko, Grzegorz Bełżecki, Anna Kasperowicz, Tadeusz Michałowski
Keywords
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