Isolation and purification of lactoferrin from colostrum of Malabari goats
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Lactoferrin, an iron-binding glycoprotein, primarily present in milk, has been shown to be involved in various physiological and protective function including roles in iron homeostasis, cell proliferation, antibacterial, antifungal, antiviral, antioxidant, immunomodulatory and anticancer activities. The present study focused on the isolation and purification of lactoferrin of Malabari goats, an indigenous goat breed of Kerala. Lactoferrin is isolated from colostrum samples by cation exchange chromatography using CM Saphadex C-50 column. The isolated protein was identified and confirmed by SDS-PAGE in terms of its molecular weight. A single 80 kDa Coomassie Brilliant Blue-stained band observed in the electrophoretic profile ascertained the purity of the protein isolated. The results of the study point to a simple one step method to obtain pure lactoferrin from goats.
Authors and Affiliations
Sinchu Vijayan, Uma Radhakrishnan, Linu Eldho, Jayavardhanan KK
Keywords
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- EP ID EP428119
- DOI 10.18006/2017.5(4).550.555
- Views 71
- Downloads 0
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