Isolation and Screening of Cellulase Producing Microorganisms from Degraded Wood
Journal Title: International Journal of Pharmaceutical and Biological Sciences Fundamentals (www.ijpbsf.com) - Year 2012, Vol 2, Issue 1
Abstract
The main objective of this study is to isolate the novel Cellulase producing microorganisms. The most potent enzyme producing strain is fungi. Cellulase production from fungi is advantageous as the enzyme production rate is higher as compared to other microorganism. For this study soil samples were collected from wood and cellulosic waste collected from dumping areas near construction sites. Microorganisms were isolated using Potato dextrose agar medium. After this screening for potent cellulase producing microorganism was carried out and the microorganisms were identified using various morphological study and biochemical tests. The enzyme activity was determined by screening the isolates for cellulolytic property. The isolates were maintained on PDA slants until needed. The microorganisms were cultured for production of cellulose in a shake flask culture. Crude enzyme was extracted and purified by centrifugation. Based on the report it was concluded that Penicillium chrysogenum can be a potential microorganism for the production of cellulase and showed maximum enzyme activity of 2.037 U/ml/min..
Authors and Affiliations
Shelly Rana , Manjeet Kaur
Keywords
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