Abstract

The objective of this experiment was to isolate protease producing microorganisms from soil samples collected from fertile agricultural land. Totally seven isolates were isolated from the soil samples by serial dilution technique, among them isolate 1 was selected for the experiment since it showed the maximum activity. The activity of protease was estimated by measuring the liberated amino acids from the protease by titration method in liquid broth of skimmed broth, also by visualizing the zone of degradation of casein from the plated skimmed agar plates. Further the isolate was studied for its culture characteristics, staining for its identification and purification of the enzyme was also done. Based on the biochemical and colony morphological study it was identified as gram positive cocci bacteria. This isolate was further studied with different parameters such as, effect of temperature, pH, nitrogen and metal ions. The optimum conditions of various parameters mentioned above were estimated. Key word: Bacterial protease, purification, industrial importance of protease

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