Development and Validation of Analytical Method for Simultaneous Estimation of Cefixime and Ofloxacin in Bulk and Tablet Dosage Form by RP-HPLC Method
Journal Title: International Journal of Pharma Research and Health Sciences - Year 2014, Vol 2, Issue 6
Abstract
The objective of present work was to develop and validate a simple, accurate,precise HPLC method for the estimation of cefixime and ofloxacin. The chromatographic separation was achieved on a Hypersil BDSC18 column (4.6x250 mm,5μmparticlesize). Different mobile phase systems in different proportions were tried. For HPLC method a mobile phase consisting of Methanol and Water (70:30) produced symmetric peak shape with good resolution for both the drugs. Next, the drugs were chromatographed under different flow rates from which a flow rate of 1.0 ml/min was selected. The retention times of cefixime and ofloxacin were found to be 2.96 min and 4.15 min, respectively.The proposed method was found to have excellent linearity in the concentration range of 20- 80mg/ml with correlation coefficient r2=0.999 and 0.999 for cefixime and ofloxacin repectively.The method was validated for linearity, precision, LOD, LOQ and robustness.The proposed method optimized and validated as per ICH guidelines.
Increasing Trends of Leptospirosis in Tamilnadu: A Clinical Epidemiological Study – with Special Emphasis of Molecular Surveillance and Diagnosis
Objective: Leptospirosis is a contemporary eco-zoonotic disease; humans get accidental infections while exposed to the contaminated environment of by animals where symptoms of this disease observed from multiorgan dysfun...
Herbal Hydrogel for Wound Healing: A Review
Wounds are inescapable events of life, which arise due to physical or chemical injury or microbial infections. Wound healing is a complex and protracted process of tissue repair and remodeling in response to injury. As p...
Chemical Composition, Antimicrobial Activity of Essential oil and Chromosome Number of Senecio jacobaea L. from Algeria
The objective of this work is to identify the chemical composition of the essential oil of Senecio jacobaea from Algeria, to test its antimicrobial activities and to identify its chromosomal number. The analysis and iden...
Epidemiological Distribution and Data Analysis of Facial Talon Cusp: A Comprehensive Review of the Literature
Objective: The purpose of the present study was to perform a comprehensive literature search on facial talon cusps to evaluate the characteristics and distribution. Methods: A review of the articles published in English...
GC-MS Analysis of Bioactive Components of Petiveria alliacea L. Whole Plant (Phytolaccaceae)
The investigation was carried out to determine the phytocompounds of ethanol extract of Petiveria alliacea whole plant. GC-MS analysis of ethanol extract was performed using a Perkin-Elmer GC clarus 500 system and Gas Ch...