Development and Validation of Analytical Method for Simultaneous Estimation of Cefixime and Ofloxacin in Bulk and Tablet Dosage Form by RP-HPLC Method
Journal Title: International Journal of Pharma Research and Health Sciences - Year 2014, Vol 2, Issue 6
Abstract
The objective of present work was to develop and validate a simple, accurate,precise HPLC method for the estimation of cefixime and ofloxacin. The chromatographic separation was achieved on a Hypersil BDSC18 column (4.6x250 mm,5μmparticlesize). Different mobile phase systems in different proportions were tried. For HPLC method a mobile phase consisting of Methanol and Water (70:30) produced symmetric peak shape with good resolution for both the drugs. Next, the drugs were chromatographed under different flow rates from which a flow rate of 1.0 ml/min was selected. The retention times of cefixime and ofloxacin were found to be 2.96 min and 4.15 min, respectively.The proposed method was found to have excellent linearity in the concentration range of 20- 80mg/ml with correlation coefficient r2=0.999 and 0.999 for cefixime and ofloxacin repectively.The method was validated for linearity, precision, LOD, LOQ and robustness.The proposed method optimized and validated as per ICH guidelines.
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