Development and Validation of Analytical Method for Simultaneous Estimation of Cefixime and Ofloxacin in Bulk and Tablet Dosage Form by RP-HPLC Method
Journal Title: International Journal of Pharma Research and Health Sciences - Year 2014, Vol 2, Issue 6
Abstract
The objective of present work was to develop and validate a simple, accurate,precise HPLC method for the estimation of cefixime and ofloxacin. The chromatographic separation was achieved on a Hypersil BDSC18 column (4.6x250 mm,5μmparticlesize). Different mobile phase systems in different proportions were tried. For HPLC method a mobile phase consisting of Methanol and Water (70:30) produced symmetric peak shape with good resolution for both the drugs. Next, the drugs were chromatographed under different flow rates from which a flow rate of 1.0 ml/min was selected. The retention times of cefixime and ofloxacin were found to be 2.96 min and 4.15 min, respectively.The proposed method was found to have excellent linearity in the concentration range of 20- 80mg/ml with correlation coefficient r2=0.999 and 0.999 for cefixime and ofloxacin repectively.The method was validated for linearity, precision, LOD, LOQ and robustness.The proposed method optimized and validated as per ICH guidelines.
Evaluation of Wound Healing Activity of Ageratina adenophora (Spreng.) R.M.King & H.Rob.
Objective: ‘Basinga’ is traditionally used as wound healing plant in Uttarakhand, India and this study was design to evaluate the wound healing potential of Ageratina adenophora for establishment of its traditional claim...
Formulation and Evaluation of Beta-Cyclodextrin Complex Tablets of Ibuprofen
The objective of present study is to prepare complexes of ibuprofen with Cyclodextrin in molar ratio by co-precipitation method. The inclusion complexes was further formulated into tables by direct compression technique...
Genes in Serious Adverse Drug Reactions
Since long it has been postulated that various ADRs have some genetic determinants.To the date various genes have been identified and studied. Presence of particular gene determines individual susceptibility for adverse...
In-Vitro Study on Spasmolytic Activity of Calotropis Procera In Rabbit Jejunum
In ancient India many herbal plant products were used for their medicinal values. Calotropis procera known as arka, madar, milkweed. It was used as antimicrobial, antifertility, anticancer, leprosy and antihelmintic. In...
Pharmacological study to evaluate acute eye irritation/corrosion activity of Vasanjana - an Ayurvedic formulation
Intramuscular fat from animal origin can be used for effective and better management of dry eye syndrome. Vasanjana is one of such Ayurvedic ocular preparation for topical use in dry eye of human beings. Present study wa...