Isolation gidazepam and its metabolites by solid-phase extraction
Journal Title: Фармацевтичний журнал - Year 2016, Vol 2, Issue 2
Abstract
Gidazepam as benzodiazepine derivative is drugs of abuse and is object of toxicological research. The first phases of analysis of analite is its insulating from biological objects. In a case of gidazepam such analites is its metabolites. One of insulating method which used in analytical toxicology is the method of solid-phase extraction (SPE). This method have advantage in comparison with is liquid extraction. However papers about studying of insulating efficiency gidazepam and its metabolites of SPE are absent now. Thus the purpose of the this paper is a study of applications of SPE in analytical toxicology. For work SPE columns Bond Elut Certify have been used (volume 3 mL, amount of a sorbent 130 mg), production of Agilent Technologies. The SPE protocols which studying have been optimised under these columns for extraction from blood and urine. Two procedures are developed for extraction in case of the general screening of an unknown drug, and two for screening of benzodiazepines. Showed that degree of extraction of the basic gidazepam`s metabolites compounds 92–98%, and for gidazepam 51–74%. Also it is positioned that acetonitrile in solutions for removal coextractive substance considerably depresses degree of extraction one of gidazeam`s metabolite. At the same time application of 1 М acetic acid promotes retention of gidazepam and its metabolites on a SPE column in the course of removal lipophilic impurities by organic solvents. Position of gidazepam and its metabolites in the schema of the general screening of an unknown drug in both SPE screening procedures is showed.
Authors and Affiliations
M. A. Savchenko
Keywords
Related Articles
- EP ID EP563109
- DOI 10.32352/0367-3057.2.16.02
- Views 64
- Downloads 0
Order of medicines rationing with the provision of qualified surgical help to military servicing
The standardization of medicines, taking into account the considerable experience of the military medical service, is a promising direction for improving the medical supply of the health facilities of the Ministry of Def...
Determination of amino acids content in the flowers of the sorts of chrysanthemum (Chrysanthemum xhortorum Baileyl.)
Amino acids play important roles in the human body. They are the building units of proteins. The aim of our researches was to study amino acids content of the flowers of Chrysanthemum sorts as Finos, Grandeur, Apro, Bel...
Cariesprophylactic and periodontoprotective action of octenidine hexafluorosilicate in rats obtained with cariesogenic ration
In recent years, ammonium hexafluorosilicate and hexafluorosilicates of organic amonium cations are actively studied, which demonstrate certain advantages over traditional compounds of fluorine. Previously, it was shown...
Antiseptic properties of aminoadamantane derivative
Development of microbial resistance to current antimicrobial drugs created a critical need of the new antiseptics. The object of our study was phenyladamantane derivative (4-(adamantyl-1)-1-(1-aminobutyl)benzol, AM-166)....
Motivation of obtaining higher pharmaceutical education of students of the National Pharmaceutical University
Choice of profession is the main factor in the further life of man, his attitude to himself and the world. When choosing a profession, a person is guided by a number of motives. In modern society, the motives of high sc...