Isolation, identification of Phytophthora nicotianae var. parasitica and screening of tomato parental lines for buckeye rot resistance

Journal Title: Journal of Applied and Natural Science - Year 2017, Vol 9, Issue 1

Abstract

Buckeye rot disease of tomato which is one of the most devastating diseases of tomato crop is caused by soil born fungus Phytophthora nicotianae var. parasitica. In present study, the pathogen was isolated, morphologically identified and its pathogenicity was proved on susceptible commercial variety Solan Lalima and resistant line EC-251649 of tomato. Isolation of pathogen from the infected tomato fruit was achieved on Corn Meal Agar (CMA) out of two different media viz., Potato Dextrose Agar (PDA) and CMA. Fungal inoculum was prepared on Corn Meal broth. Inoculation with 10 ml of inoculum was found optimum in plant parts namely, stem, leaves and fruits. Parental lines were inoculated to test their disease reaction to buckeye rot. Symptoms of infection appeared on leaves and fruits only. Solan Lalima was found to be highly susceptible to with disease severity of 92 % and 100 % disease incidence, while EC-251649 was found moderately resistant on the basis of 16 % disease severity and 10 % disease incidence to the disease. After confirmation of resistance and susceptibility, the parental lines were surveyed for polymorphism using 42 primers and 32 were recorded to be polymorphic revealing that the differences are present at DNA level also. This is the very first study which evaluated parental lines for buckeye rot disease reaction on morphological as well as molecular basis. These lines will be further used for quantitative trait loci (QTL) analysis/gene tagging for buckeye rot and marker assisted selection to provide improved varieties to the farmers.

Authors and Affiliations

Shilpa Shilpa, Rajinder Kaur, Monica Sharma, M. N. Adarsh

Keywords

Related Articles

Biocontrol of toxigenic strain of Aspergillus flavus isolated from the root tubers of safed musli (Chlorophytum borivilianum Sant. F) using its rhizospheric mycoflora

Miraculous herb safed musli (Chlorophytum borivilianum Sant. F), family liliaceae, is well recognized for its immense potential as an aphrodisiac. The root tubers of this herbal drug were found to be invested with Asperg...

Toxicological studies on Helicoverpa armigera in pigeonpea growing in Vidarbha region of Maharashtra, India

Insecticide resistance level in pigeonpea pod borer, Helicoverpa armigera (Hubner) to technical grade insecticides collected from major pigeonpea growing districts of Vidarbha viz., Akola, Amravati, Buldhana, Yavatmal an...

Description of a new species Cephaline Gregarine Stenophora bristili (Apicomplexa, Sporozoea) from Millipede (Chondromorpha severini) in Aurangabad district (M.S), INDIA

The study of the endoparasitic cephaline gregarine in the gut content of millipede (Chondromorpha severini) was found to be infested with a new species (Stenophora bristili) of genus Stenophora (Labee, 1899). It differs...

Antioxidative role of selected herbs against ethanol induced liver injury in rats

The purpose of this study was to know the hepatotoxi City of ethanol in laboratory rats Rattus rattus and to observe the individual and combined phytotherapeutic role of five herbs viz.Arctium lappa, Curcuma longa, Piper...

Extractability and availability index of sulphur in selected soils of Odisha

We aimed to evaluate the extractability of two reagents viz.0.15% CaCl2 & 0.01M Ca (H2PO4) for sulphur along with the sulphur availability index of mixed red and black soils. For this 86 soil samples were collected from...

Download PDF file
  • EP ID EP286612
  • DOI 10.31018/jans.v9i1.1230
  • Views 103
  • Downloads 0

How To Cite

Shilpa Shilpa, Rajinder Kaur, Monica Sharma, M. N. Adarsh (2017). Isolation, identification of Phytophthora nicotianae var. parasitica and screening of tomato parental lines for buckeye rot resistance. Journal of Applied and Natural Science, 9(1), 562-567. https://europub.co.uk/articles/-A-286612