Abstract

Objective: Aim of present study to evaluate anticancer activity of dried fruit of Embelia ribes containing embelin in various types of cancer. Material and Method: Embelin was extracted and isolated from Embelia ribes berries of Indian origin. Isolated embelin was characterized by melting point, TLC, partition coefficient, solubility, FT-IR, NMR and Mass spectroscopy. Assessment of anticancer activity was done by Cytotoxicity assay and Apoptosis assay by using MCF‑7 breast cancer cells. Isolated Embelin was investigated on MCF-7 breast cancer cell line by MTT assay and apoptosis assay. Different concentration of embelin were used and observed that 80μg/ml was the IC50 value of embelin in MCF‑7 breast cancer cells and it was found that embelin was able to affect the viability of cells and also induce apoptosis of MCF‑7 breast cancer cells in a dose dependent manner. Results: We have found that the isolated embelin have significant (٭p < 0.0001) potency against the MCF-7 breast cancer cells in dose dependent manner and showed significant increase in apoptotic cells at IC50 value. Conclusion: Isolated embelin have significant potency against the MCF-7 breast cancer cells in dose dependent manner.

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