ISOLATION OF NOVEL ORGANISM STENOTROPHOMONAS KOREENSIS strain AK-1 WITH POTENTIAL OF PAHs DEGRADATION.
Journal Title: GJRA-Global Journal For Research Analysis - Year 2015, Vol 4, Issue 5
Abstract
PAHs are an environmental concern because of their reduced bio-availability. Biodegradation via microorganisms who possessrequiredenzymesystemsforthebreakdownofrecalcitrantcompoundsisoneofthebestoptionsavailable, astheend products are non toxic. Stenotrophomonas koreensis AK-1(GENBANK:KP145677.1) a novel organism, was isolated from dust on dark leaves of Ficus religiosa ( Peepal tree) ,which could aerobically degrade various aromatichydrocarbons(Phenol,Benzene,Aniline,Xylene,Anthracene,Naphthalene). The degradation was estimated by analytical techniques like U.V visible spectroscopy, Reverse Phased HPLC and Phenol estimation titration. The key feature of the organism was its ability to degrade phenol and phenolic compounds aerobically. It was observed that genes for enzyme complex required for degradation were constitutive and not plasmid borne, as no plasmids were observed on Agarose gel electrophoresis. The organism, thus, holds potential for further application in field of biodegradation of PAHs.
Authors and Affiliations
Amruta Kothare, Aditi Ghosh, Prabha Padmanabha
Keywords
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