Isolation of Rare Salmonella Serovars, Wangata and Penarth from Chicken in Nsukka, Nigeria
Journal Title: Microbiology Research Journal International - Year 2016, Vol 16, Issue 1
Abstract
Introduction: Salmonella infections remain a veterinary and public health problem of major importance. Rare Salmonella serovars, whose epidemiological and serological patterns are not well understood, are becoming increasingly common in Nigeria and other parts of the world. We report the isolation of Salmonella enterica serovars Wangata and Penarth, two serovars that had not been previously reported in chicken in Nigeria. Materials and Methods: A total of 300 chickens comprising of 150 intensively reared and 150 free range chickens, from selected farms and live bird markets, were sampled by cloacal swab using International Office of Epizootics (OIE) standards. Following standard bacteriological techniques, samples were pre-enriched in buffered peptone water, before transferring into Rappaport Vassiliadis medium and finally streaked onto Salmonella-Shigella agar (SSA). Salmonella spp. were identified biochemically and serotyped based on reaction with somatic (O), flagella (H), and capsular (Vi) antisera. Antibiotic susceptibility testing was performed following Kirby-Bauer (disk-diffusion) method. Results: Out of the 300 samples, 4% (n = 12) were positive for salmonellae. The isolates comprise of 6 isolates of S. enterica ser Wangata, 5 S. enterica ser Enteritidis and 1 S. enterica ser Penarth. All the rare serotypes S. Wangata and S. Penarth were isolated from free range chickens, while S. Enteritidis was isolated from both intensively reared and free range chickens. There was no difference in the sensitivity pattern between the rare serovars and serovar Enteritidis to the antibiotics tested. S. Penarth had a higher MIC to Cotrimoxazole, but lower MBC for gentamicin and tetracycline. Conclusions: Free range chickens could be vehicles for the transmission and/or reservoirs of the rare salmonellae serotypes in Nigeria. Any prophylactic program aimed at controlling these agents in poultry farms in Nigeria, must take into account the free range local chickens.
Authors and Affiliations
Okechukwu J. Obi, Anthony C. Ike, Chinasa V. Olovo
Keywords
Related Articles
- EP ID EP351960
- DOI 10.9734/BMRJ/2016/27849
- Views 119
- Downloads 0
Antibiotics Susceptibility Profile of Extended Spectrum Beta Lactamase Producing Gram Negative Bacteria from Widal Positive Patients in Ekiti State University Teaching Hospital
Aims: To investigate the age occurrence of typhoid fever, antibiotics susceptibility profile of Extended Spectrum Beta-lactamase producing gram negative bacteria recovered from the blood culture of widal positive patient...
Serological Detection of Equine Herpes Virus (EHV) Type 1 and 4 in Sudan
Aims: This study was designed to investigate the presence of EHV 1 & 4 in blood serum of horses and donkeys in different localities in Sudan. Study Design: A total of 208 blood serum samples were collected from horse an...
The Prevalence of Vaginal Candida Colonization among Female Students in Bingham University
Aim: To determine the prevalence of vaginal Candida colonization among female students in Bingham University. Place and Duration of Study: This study was carried out in Bingham University, Auta balefi, Karu LGA, Nasaraw...
Identiļ¬cation and Characterization of Lactobacilli from Rajshahi Traditional Curds
The popular food, dairy products like curds are consumed by many people in its various forms in Bangladesh. Infact, these different types of curds are made without real respect for basic standards of technology and hygie...
Diversity of Plasmid Profile in Multi-drug Resistant Non- E. coli Intestinal Flora Lacking Association with Resistance Phenomenon
Aims: To study the plasmid diversity of intestinal non- Escherichia coli community, describe their potential as a reservoir of antibiotic resistance phenomenon and to find any association of resistance phenomenon with th...