Isolation of Rare Salmonella Serovars, Wangata and Penarth from Chicken in Nsukka, Nigeria
Journal Title: Microbiology Research Journal International - Year 2016, Vol 16, Issue 1
Abstract
Introduction: Salmonella infections remain a veterinary and public health problem of major importance. Rare Salmonella serovars, whose epidemiological and serological patterns are not well understood, are becoming increasingly common in Nigeria and other parts of the world. We report the isolation of Salmonella enterica serovars Wangata and Penarth, two serovars that had not been previously reported in chicken in Nigeria. Materials and Methods: A total of 300 chickens comprising of 150 intensively reared and 150 free range chickens, from selected farms and live bird markets, were sampled by cloacal swab using International Office of Epizootics (OIE) standards. Following standard bacteriological techniques, samples were pre-enriched in buffered peptone water, before transferring into Rappaport Vassiliadis medium and finally streaked onto Salmonella-Shigella agar (SSA). Salmonella spp. were identified biochemically and serotyped based on reaction with somatic (O), flagella (H), and capsular (Vi) antisera. Antibiotic susceptibility testing was performed following Kirby-Bauer (disk-diffusion) method. Results: Out of the 300 samples, 4% (n = 12) were positive for salmonellae. The isolates comprise of 6 isolates of S. enterica ser Wangata, 5 S. enterica ser Enteritidis and 1 S. enterica ser Penarth. All the rare serotypes S. Wangata and S. Penarth were isolated from free range chickens, while S. Enteritidis was isolated from both intensively reared and free range chickens. There was no difference in the sensitivity pattern between the rare serovars and serovar Enteritidis to the antibiotics tested. S. Penarth had a higher MIC to Cotrimoxazole, but lower MBC for gentamicin and tetracycline. Conclusions: Free range chickens could be vehicles for the transmission and/or reservoirs of the rare salmonellae serotypes in Nigeria. Any prophylactic program aimed at controlling these agents in poultry farms in Nigeria, must take into account the free range local chickens.
Authors and Affiliations
Okechukwu J. Obi, Anthony C. Ike, Chinasa V. Olovo
Keywords
Related Articles
- EP ID EP351960
- DOI 10.9734/BMRJ/2016/27849
- Views 87
- Downloads 0
Detection of Carbapenem Resistance in Salmonella Species from a Tertiary Hospital in Eastern Cape, South Africa
Aims: Broad-spectrum carbapenem group is the current therapy for strains of Enterobacteriaceae that express extended spectrum beta-lactamases (ESBLs). However, recent reports of therapeutic failures of carbapenems with s...
Animal Trypanosomosis in Kaura Local Government Area, Kaduna State, Nigeria
A survey of animal trypanosomosis in Kaura LGA of Kaduna State was undertaken. Blood samples were collected from 635 animals in five Districts of the LGA. The blood samples collected from different species comprising of...
Bacteriological Assessment of Hawked Sorrel Drink (Zobo Drink) in Aba, South-East Nigeria
Bacteriological investigation of hawked Sorrel drink (zobo drink) was carried out in Aba, South-east Nigeria, between January and March, 2014. Zobo drink is a non-alcoholic local beverage made from different varieties of...
Antimicrobial Potential of Dacryodes edulis against Selected Clinical Bacterial Isolates
The antimicrobial potential of Dacryodes edulis was investigated to determine the antimicrobial properties of the collected raw D. edulis pulp and seed extracts on some medically important human pathogens. The research w...
In vitro Assessment of the Antibacterial Activity of Matricaria chamomile Alcoholic Extract against Pathogenic Bacterial Strains
An alarming increasing in the occurrences of antimicrobial resistance inside the existing clinical use and so the recent appearance of multidrug resistant bacteria that attempts the treatment of infections necessities to...