Karakterisasi Kromosom Stroberi (Fragaria vesca L. subsp. californica Cham. & Schltdl. cv. Californica) Hasil Poliploidisasi
Journal Title: Biogenesis: Jurnal Ilmiah Biologi - Year 2015, Vol 3, Issue 2
Abstract
Fruit consumption such as strawberries always increase in Indonesia and reaching about 210 tons in 2011. Strawberry (Fragaria vesca L. subsp. californica Cham. and Schltdl. cv. Californica) has been developed in Indonesia, especially in Banyuroto, Sawangan, the district of Magelang, Central Java. In order to improve the fruit productivity (quality and quantity), genetic characterization including chromosomes character of this fruit are needed to be investigated. Therefore, the research was conducted to determine the chromosomes number, chromosomes size, chromosomes shape and karyotype of control and strawberries treated by colchicine as mutagenic agent. The method was squash and flow cytometry method. Whereas the cell suspension sample were observed used flow cytometer instrument. The result of research shown that the chromosome number of strawberry Californica in normal and treatment strawberries was 2n=14. The chromosomes shape of normal strawberry was 10 metacentric and 4 submetacentric. Whereas the chromosomes shape of treatment strawberries used colchicine was 10 metacentric and 4 submetacentric. Flow cytometric analysis shown that control strawberries and treated strawberries has diploid ploidy level.
Authors and Affiliations
Rosyidatul Khoiroh, Ganies Riza Aristya, Sutikno Sutikno, Niken Satuti Nur Handayani
Keywords
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- EP ID EP300170
- DOI 10.24252/bio.v3i2.932
- Views 200
- Downloads 0
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