LABORATORY DIAGNOSIS OF HELICOBACTER PYLORI INFECTION IN ENDOSCOPIC BIOPSY SAMPLES OF DYSPEPTIC PATIENTS- A HOSPITAL-BASED DIAGNOSTIC ACCURACY STUDY
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 52
Abstract
BACKGROUND The identification of the clinical consequences of Helicobacter pylori (H. pylori) infection is certainly one of the major discoveries within the past 20 years in medicine. Dyspepsia is a very common group of symptom referring to upper gastrointestinal tract for which patients consult the physician, accounting for about 4% to 14% of their consultations. Annual Incidence of Helicobacter pylori is 6%-14% in developing countries with faeco-oral route of transmission. In this context, this study has been undertaken to look for the association of H. pylori in adult patients of dyspepsia with comparative evaluation of diagnostic test for detection of Helicobacter pylori in dyspeptic patients visiting in a tertiary hospital in a rural setup of central India. MATERIALS AND METHODS It was a cross sectional study involving 52 patients. After obtaining history and written informed consent, 4 antral mucosal biopsy specimens were collected along with 5 mL blood sample. Biopsy specimens were subjected to rapid urea test, culture in Brucella broth, Gram staining and histopathological examination. subculture from Brucella broth was done on Brucella chocolate agar with & without antibiotics. Identification was done by standard tests. Serum was separated and used for ELISA test for H. pylori IgG antibody. RESULTS Out of the 52 patients studied, 25 (48.07%) were positive by Rapid Urease Test (RUT), 20 (38.46%) by Gram staining, 17 (32.69%) by Culture, 21 (40.38%) by Histopathology, and 28 (53.84%) by H. pylori IgG antibody by ELISA test. RUT had 100% sensitivity and 77.14% specificity, while histopathology had 94.11% sensitivity and 85.14% specificity and H. pylori IgG antibody had 94.11% sensitivity and 65.71% specificity in reference to culture. CONCLUSION In our study, we have revealed that for diagnosing of H. pylori one can use RUT for rapid diagnosis in the endoscopic room itself and confirm diagnosis by culture.
Authors and Affiliations
Kumar Ratnesh, Deotale Vijayashri, Jain Jyoti, Gangane Nitin, Mendirata Kr Deepak, Narag Pratibha
Keywords
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- EP ID EP220202
- DOI 10.14260/Jemds/2017/855
- Views 118
- Downloads 0
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