Large-Scale Deletion of Non-Essential Genes Region of Chromosome XV in Saccharomyces Cerevisiae
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 3
Abstract
We firstly applied ‘Latour system’ to Saccharomyces cerevisiae for large-scale deletion, and ~38 kb target region (nt 143376 -181682) of chromosome XV was deleted precisely and efficiently with no foreign sequences left. There are 20 ORFs in the target region, including ars1510, a chromosomal replication origin. The result demonstrates that this method is a useful tool for large-scale chromosomal modification, and for reducing the genome of S. cerevisiae to obtain the minimal cell in synthetic biology. More than that, this method is applicable for further study of analyzing functions and interactions of clusters of genes.
Authors and Affiliations
Xunxun Shi1, Hai Wu, Feijie Li3, Hui Tang, Liping Zhang*
Keywords
Related Articles
- EP ID EP384574
- DOI 10.9790/3008-1103034650
- Views 73
- Downloads 0
Determinants of Alcohol Consumption among Tertiary education Students in Port Harcourt
Alcohol is a known substance in the society and readily abused. The consumption of alcohol has continued to increase among students in various institutions of learning across the world. This research work was carried out...
Effects of Temperature, pH, and Agitation Rate on the Production of Microbial L-Arginase
The enzyme arginase metabolizes L-arginine to L-ornithine and urea. Besides its fundamental role in the hepatic urea cycle, arginase is also expressed the immune system of mice and man. While significant interspecies dif...
Medicinal Plants Used By Traditional Healers in UNA District of Himachal Pradesh (North Western Himalayan Region), India
The present study was conducted in a Swan river (Main River of district) catchment area of district Una (H.P) to accumulate knowledge regarding diversity of plant resources that are used by local people for medicinal use...
Antimicrobial, Antioxidant Properties And Phytochemical Analysis Of Opilia Amentaceae
Plants produce thousands of specialized metabolites, many of which have medicinal uses. Over the next few years, the study of medicinal plants as antimicrobial agents should be focused on ascertaining specific informatio...
Influence of Various Dosage Forms on Stability and Corneal Bioavailability of Desloratadine Ophthalmic Preparations
Designing ophthalmic drug delivery system to a target tissue of the eye has become a major challenge for scientists. The aim of the present study was to formulate desloratadine (Des) in different ophthalmic preparations...