Abstract

The Liquid Chromatography-Mass Spectrometry (LC-MS) is a powerful analytical technique with very high sensitivity and specificity. LC-MS is combination of Liquid Chromatography (LC) and Mass Spectrometry (MS). With the Liquid Chromatography (LC) the separation of components can be done and then the sample eluents from LC are transferred into Mass Spectrometry (MS) where the detection, identification and determination of masses of components can be done in presence of other components. LC-MS is used in determination, of pharmaceutical drug substances, intermediates and its related compounds for quantitative and qualitative purpose. LC-MS is used most significantly in in-vitro dissolution, bio-equivalence, bioavailability and metabolite studies. Also LC-MS is used in basic research, agrochemical, forensic laboratories and food industries .In this article principle of LC-MS, instrumentation and its applications are briefly discussed.

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