<strong>Biochemical and Molecular Characterization of Carotenogenic Flavobacterial Isolates from Marine Waters</strong>
Journal Title: Polish Journal of Microbiology - Year 2016, Vol 65, Issue 1
Abstract
Carotenoids are known to possess immense nutraceutical properties and microorganisms are continuously being explored as natural source for production of carotenoids. In this study, pigmented bacteria belonging to [i]Flavobacteriaceae[/i] family were isolated using kanamycin-containing marine agar and identified using the molecular techniques and their phenotypic characteristics were studied along with their potential to produce carotenoids. Analysis of random amplification of polymorphic DNA (RAPD) banding patterns and the fragment size of the bands indicated that the 10 isolates fall under two major groups. Based on 16S rRNA gene sequence analysis the isolates were identified as [i]Vitellibacter[/i] sp. (3 isolates), [i]Formosa[/i] sp. (2 isolates) and [i]Arenibacter[/i] sp. (5 isolates). Phenotypically, the isolates showed slight variation from the reported species of these three genera of [i]Flavobacteriaceae[/i]. Only the isolates belonging to [i]Vitellibacter[/i] and [i]Formosa[/i] produced flexirubin, a typical yellow orange pigment produced by most of the organisms of the family [i]Flavobacteriaceae[/i]. [i]Vitellibacter[/i] sp. and [i]Formosa[/i] sp. were found to produce higher amount of carotenoids compared to [i]Arenibacter[/i] sp. and zeaxanthin was found to be the major carotenoid produced by these two species. The study indicated that [i]Vitellibacter[/i] sp. and [i]Formosa[/i] sp. can be exploited for production of carotenoids, particularly zeaxanthin.
Authors and Affiliations
Rama Sowmya, Nakkarike M. Sachindra
Keywords
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