An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices
Journal Title: Polish Journal of Microbiology - Year 2017, Vol 66, Issue 2
Abstract
Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.
Authors and Affiliations
Angélica Godínez-Oviedo, Gerardo M. Nava, Sofía M. Arvizu-Medrano, Montserrat Hernández-Iturriaga
Keywords
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- EP ID EP251333
- DOI 10.5604/01.3001.0010.7869
- Views 86
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