<strong>Enzymes Involved in Naproxen Degradation by <em>Planococcus</em> sp. S5</strong>
Journal Title: Polish Journal of Microbiology - Year 2016, Vol 65, Issue 2
Abstract
Naproxen is a one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) entering the environment as a result of high consumption. For this reason, there is an emerging need to recognize mechanisms of its degradation and enzymes engaged in this process. [i]Planococcus[/i] sp. S5 is a gram positive strain able to degrade naproxen in monosubstrate culture (27%). However, naproxen is not a sufficient growth substrate for this strain. In the presence of benzoate, 4-hydroxybenzoic acid, 3,4-dihydroxybenzoic acid or vanillic acid as growth substrates, the degradation of 21.5%, 71.71%, 14.75% and 8.16% of naproxen was observed respectively. It was shown that the activity of monooxygenase, hydroxyquinol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and protocatechuate 4,5-dioxyegnase in strain S5 was induced after growth of the strain with naproxen and 4-hydroxybenzoate. Moreover, in the presence of naproxen activity of gentisate 1,2-dioxygenase, enzyme engaged in 4-hydroxybenzoate metabolism, was completely inhibited. The obtained results suggest that monooxygenase and hydroxyquinol 1,2-dioxygenase are the main enzymes in naproxen degradation by [i]Planococcus[/i] sp. S5.
Authors and Affiliations
Danuta Wojcieszyńska, Dorota Domaradzka, Katarzyna Hupert-Kocurek, Urszula Guzik
Keywords
Related Articles
- EP ID EP81078
- DOI -
- Views 84
- Downloads 0
Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin
The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aure...
Prevalence of Parasitic Contamination in Salad Vegetables Collected from Supermarkets and Street Vendors in Amman and Baqa’a – Jordan
One of the main ways in transmitting parasites to humans is through consuming contaminated raw vegetables. The aim of this study was to evaluate the prevalence of parasitological contamination (helminthes eggs, [i]Giardi...
The Functions of Effector Proteins in Yersinia Virulence
[i]Yersinia [/i]species are bacterial pathogens that can cause plague and intestinal diseases after invading into human cells through the Three Secretion System (TTSS). The effect of pathogenesis is mediated by [i]Yersin...
PCR Detection of Scopulariopsis brevicaulis
[i]Scopulariopsis brevicaulis[/i] is known as a most common etiological factor of the mould toenail infections. There are also reports indicating that [i]S. brevicaulis[/i] could cause organ and disseminated infections....
Prevalence of Urogenital Mycoplasmas Among Men with NGU in Upper Silesia, Poland. Preliminary Study.
The prevalence of urogenital mycoplasmas in men with NGU in Upper Silesia (Poland) was studied. Mycoplasmas were detected in 36.7% men ([i]Ureaplasma parvum[/i] and [i]Mycoplasma genitalium[/i] were found in 30% and 1...