PCR Detection of Scopulariopsis brevicaulis
Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 1
Abstract
[i]Scopulariopsis brevicaulis[/i] is known as a most common etiological factor of the mould toenail infections. There are also reports indicating that [i]S. brevicaulis[/i] could cause organ and disseminated infections. Nowadays microscopic observations from the specimen and culture are crucial for the appropriate recognition of the infection. In this paper a new PCR-based method for [i]S. brevicaulis[/i] detection is presented. The specificity of the reaction was confirmed, as positive results were obtained only for tested DNA extracted from [i]S. brevicaulis[/i] isolates and no positive results were obtained for DNA from 57 moulds, 65 dermatophytes, 30 yeast-like fungi, and human DNA.
Authors and Affiliations
Milena Kordalewska, Anna Brillowska-Dąbrowska
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