PCR Detection of Scopulariopsis brevicaulis

Journal Title: Polish Journal of Microbiology - Year 2015, Vol 64, Issue 1

Abstract

[i]Scopulariopsis brevicaulis[/i] is known as a most common etiological factor of the mould toenail infections. There are also reports indicating that [i]S. brevicaulis[/i] could cause organ and disseminated infections. Nowadays microscopic observations from the specimen and culture are crucial for the appropriate recognition of the infection. In this paper a new PCR-based method for [i]S. brevicaulis[/i] detection is presented. The specificity of the reaction was confirmed, as positive results were obtained only for tested DNA extracted from [i]S. brevicaulis[/i] isolates and no positive results were obtained for DNA from 57 moulds, 65 dermatophytes, 30 yeast-like fungi, and human DNA.

Authors and Affiliations

Milena Kordalewska, Anna Brillowska-Dąbrowska

Keywords

Related Articles

Identification and Localization of β-D-Glucosidase from Two Typical Oenococcus oeni Strains

β-D-glucosidase (βG) gene from [i]Oenococcus oeni [/i]SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. Results showed that βG gene of the two strains was...

Comparison of Methods Used for the Diagnosis of Epstein-Barr Virus Infections in Children

The accurate diagnosis of Epstein-Barr virus (EBV) infections is important, as many other infectious agents or diseases can cause similar symptoms. In this study, sera of pediatric patients who were suspected to have an...

<strong>Relationship between <em>ure</em>B Sequence Diversity, Urease Activity and Genotypic Variations of Different <em>Helicobacter pylori</em> Strains in Patients with Gastric Disorders</strong>

Association of the severity of[i] Helicobacter pylori [/i]induced diseases with virulence entity of the colonized strains was proven in some studies. Urease has been demonstrated as a potent virulence factor for [i]H. py...

Characterization of Rhizobial Bacteria Nodulating Astragalus corrugatus and Hippocrepis areolata in Tunisian Arid Soils

Fifty seven bacterial isolates from root nodules of two spontaneous legumes (Astragalus corrugatus and Hippocrepis areolata) growing in the arid areas of Tunisia were characterized by phenotypic features, 16S rDNA PCR-RF...

<strong>Characterization of Bacteria Isolation of Bacteria from Pinyon Rhizosphere, Producing Biosurfactants from Agro-Industrial Waste</strong>

Two hundred and fifty bacterial strains were isolated from pinyon rhizosphere and screened for biosurfactants production. Among them, six bacterial strains were selected for their potential to produce biosurfactants usin...

Download PDF file
  • EP ID EP59284
  • DOI -
  • Views 99
  • Downloads 0

How To Cite

Milena Kordalewska, Anna Brillowska-Dąbrowska (2015). PCR Detection of Scopulariopsis brevicaulis. Polish Journal of Microbiology, 64(1), 65-68. https://europub.co.uk/articles/-A-59284