mAb Higher Order Structure Analysis with Protein Conformational Array ELISA
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 7, Issue 6
Abstract
The clinical and biological properties of protein-based therapeutics, or biologics, are closely related to their Higher Order Structures (HOS) which in turn can be altered by many physical and chemical conditions. A novel technology to monitor changes in monoclonal antibody (mAb) HOS is the Protein Conformational Array (PCA) ELISA which uses a bank of more than 30 antibodies to measure protein epitope change on the surface of the mAb. Using this technology, this report provided interesting findings for the first time on the HOS changes in response to the various conditions often encountered during mAb formulation development. Specifically, one IgG1 and four IgG2 native molecules in formulation buffer were compared with the same IgG which had undergone exposure to increased temperature, pH extremes and light exposure. In addition, we also examined the impact of glycation and de-glycosylation on the mAb HOS. This study demonstrated that the PCA ELISA is stability-indicating and can provide detailed HOS information that could be important for the successful development of monoclonal antibodies.
Authors and Affiliations
Michael Davies, Gan Wang, Guofeng Fu, Xing Wang
Keywords
Related Articles
- EP ID EP344683
- DOI 10.9734/BJPR/2015/18952
- Views 111
- Downloads 0
Synthesis and Evaluation of Analgesic and Anti-inflammatory Activities of Most Active Antioxidant Derivatives of Embelin
Objective: To evaluate antioxidant, analgesic and anti-inflammatory properties of embelin and its derivatives. Methods: In the present study embelin was condensed with various aliphatic substituted primary amines, hydra...
Investigation of Design Principles and Users Demand for Hospital Gardens: Case Study of Egirdir-Turkey
Aims: The plants in hospital garden have many functions to users. Hence attention on those areas should be done. A specific hospital and its gardens chosen to be determine expectations and express of users to determine s...
Evaluation of Ampicillin and Ceftriaxone on Ethanol Consumption as Compared to Naltrexone in Alcohol Dependant Rats
Context: Extracellular glutamate level in reward centre of brain increases during ethanol drinking sessions. Hence, it can be hypothesized that drugs which decrease extracellular glutamate might have deaddictive properti...
Antibacterial Potency of Ethnobotanical Plants as Alternative Remedies to Curtail Nosocomial Infections: A Case Study of Five Native Plants in Kenya
Aim: The current study was done to investigate the antibacterial potentiality of five ethnobotanical plants used in Kenya as remedies in the treatment against selected nosocomial infectious bacteria. Materials: The plan...
A Study of Serum Levels of Select Heavy Metals and Histology of Brain, Lung, Heart and Ileum of Rats Chronically Exposed to Kerosene
In rural Africa, a number of alternative treatment options are explored because of poverty and unawareness. Kerosene is one such agent used for the treatment of a myriad of diseases. The aim of the study was to investiga...