Malus domestica as an Inhibitor of Glycation
Journal Title: Scholars Academic Journal of Biosciences - Year 2014, Vol 2, Issue 2
Abstract
Abstract: Diabetes mellitus is a metabolic disorder that is principally characterized by insulin resistance, relative insulin deficiency and hyperglycemia. The present research work was designed to study the inhibition of glycation with natural Inhibitor “Malus domestica”. For this purpose, normal plasma was collected from healthy volunteers. To study glycation inhibition, thirty two combinations were made and all these combinations were placed at 37oC at same time for five weeks. Human normal plasma was used as a protein. Four milliliter of samples was drawn after 1st, 2nd, 3rd, 4th and 5th week of incubation to perform the experiments. Browning production was recorded by taking absorbance at 370nm of all combinations. Dialysis was performed to remove the excess/ free glucose. Glucose and protein estimation was done to check the glucose and protein concentration before and after dialysis. Glycation inhibition was measured with TBA assay and also by ELISA. The activity of I2 (10 times diluted) inhibitor was approximately high mainly in the 2nd week of incubation. “Malus domistica” is effective and efficient in lowering the glycation level in conditions when the level of glucose is high i.e. in diabetes. So the results indicate that in future “Malus domestica” can be used for lowering glucose level in the body. Keywords: Advanced Glycation end products, Non-Insulin Dependant Diabetes Mellitus, Inhibitor, Enzyme linked Immunosorbent Assay, ThiobarbituricAcid.
Authors and Affiliations
Rizwan Ahmad, Nosheen Aslam, Munir Ahmad Sheikh
Keywords
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