Mast Cell Density in Oral Lesions using Metachromatic Stains: A Comparative Study
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 10
Abstract
Introduction: Mast Cells (MCs) are bone marrow derived granular cells, distributed throughout the body near blood vessels, nerves and subepithelial areas. MC granules stain by basic dyes but are most readily demonstrated by metachromatic dyes such as toluidine blue and azure A. Aim: This study focuses on evaluating and comparing the count of MCs by identification and staining of these cells by azure A with toluidine blue as a control, in normal oral mucosa and in various other oral pathologies. Materials and Methods: Five cases each of Normal Oral Mucosa (NOM), Inflammatory Fibrous Hyperplasia (IFH), Oral Pyogenic Granuloma (OPG), Oral Lichen Planus (OLP) and Oral Squamous Cell Carcinoma (OSCC) were stained with 1% toluidine blue and azure A. Results: Mean MC count was higher in all four lesions when compared to normal oral mucosa with both stains. OLP exhibited the maximum amount of mean MC count when compared to other pathologies under study. With all four lesions, toluidine blue exhibited higher number of MC density (count/high power field) compared to azure A. Conclusion: Higher count of MCs was noticed in all four lesions indicating a possible role of MCs in their pathogenesis either directly or indirectly. Also, the number of degranulated MCs was more in OLP followed by OSCC, IFH and OPG.
Authors and Affiliations
Shilpa Chirappurath Natesan, Jose George, Rekha Krishna pillai, Bindhu Pushparajan Ramakrishnan, Priya Thomas
Keywords
Related Articles
- EP ID EP365872
- DOI 10.7860/JCDR/2017/30460.10744
- Views 54
- Downloads 0
Universal Presence of blaNDM-1 Gene in Carbapenem-Resistant Gram-Negative Bacilli in an Indian Hospital in 2015
Letter to Editor
Fistulating Richter’s Hernia of Groin with Necrotizing Soft Tissue Infection: A Lethal Combination
Strangulation of groin hernia can result in significant morbidity and mortality. Spontaneous external fistulation following strangulation is rare and typically occurs with Richter’s hernia. Spreading Necrotizing Soft Tis...
Foreign Body Aspiration: An Unusual Presentation and Outcome
Foreign body aspiration mostly presents as acute emergency with cough, choking and dyspnoea. Rarely aspiration of foreign body may be the underlying cause in patients presenting with long term symptomatologies. Here is a...
Nasopharyngeal Pleomorphic Adenoma: A Difficult Surgical Proposition
Salivary gland tumours of the nasopharynx are rare and when present, tend to be malignant. Here, we report an unusual case of benign pleomorphic adenoma of the minor salivary gland arising from the nasopharynx in a 21-ye...
Urinary Uric Acid/Creatinine Ratio - A Marker For Perinatal Asphyxia
Background: Perinatal hypoxia is one of the leading causes of perinatal mortality in developing countries. Both apgar score and arterial blood pH predict the neonatal mortality in asphyxia. Apgar score alone does not pre...