Measurement of Forchlorfenuron in Grapes by Surface - Enhanced Raman Spectroscopy coupled with Gold Nano substrates
Journal Title: International Journal of Food Science, Nutrition and Dietetics (IJFS) - Year 2016, Vol 5, Issue 1
Abstract
The objective of this study was to use surface-enhanced Raman spectroscopy (SERS) for rapid detection and characterization of trace amounts of forchlorfenuron extracted from fruits. Forchlorfenuron is a plant growth regulator widely used in grapes and has negative effects on human health. In this study, gold-coated nanosubstrates were used for SERS measurements. Partial least squares (PLS) analysis was used as a statistical method for quantitative analysis of the spectral data. Our results demonstrate that enhanced Raman signals acquired from samples exhibited characteristic spectral patterns. The PLS results for quantification of forchlorfenuron were obtained: R=0.96, RMSEP=9.336×10-6. The detection limit for for chlorfenuron by SERS is 3.15ppm for pure forchlorfenuron solutions and 4.43 ppm for forchlorfenuron extracted from grape skin. In addition, HPLC was also used to measure forchlorfenuron and verify SERS results. A good linear relationship was observed between 0 - 100 ppm with an R value of 0.999. These results demonstrate that SERS coupled with gold nanosubstrates is a rapid and simple method and it requires little sample preparation. It could be a practical approach to combine SERS with HPLC method to screen and analyze food samples for chemical contaminants and residues.
Authors and Affiliations
Mengshi Lin
Keywords
Related Articles
- EP ID EP201896
- DOI 10.19070/2326-3350-1600046
- Views 87
- Downloads 0
Determination of Maltose in Honey
Honey is an excellent nutritious food and is widely produced and marketed. However, there have been complaints that some marketed honey is adulterated with maltose. We quantified glucose, fructose, maltose and sucrose co...
Hyperhomocysteinemia and Type 2 Diabetes in Côte d'Ivoire
The hyperhomocysteinemia is associated with the development and the accentuation of the cardiovascular complications of the type 2 diabetes. The objective of this study was to evaluate the hyperhomocysteinemia in type 2...
Standard Of Identity” For Probiotic Supplemented Foods
Standard Of Identity” For Probiotic Supplemented Foods
Quality Evaluation of Millet-Based Fura Powder Supplemented with Bambara Groundnut
“Fura”is known to have originated from Hausa/Fulani and is produced mainly from most flour, blended with species, compressed into balls and boiled for thirty minutes, while still hot, the cooked dough is worked through i...
Nutrition Module Intervention to Improve Nutrition Knowledge, Attitude and Practice of Trainers for Persons with Disabilities in Rehabilitation Centers in Malaysia
Introduction: Other than parents, primary care providers such as community workers and rehabilitation service personnel are also important nutritional gatekeepers for persons with disabilities (PWD). Good nutrition manag...