Membrane Stability and Antioxidant Activity of Gmelina arborea Seed Extracts and Their Fatty Acid Composition
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 6, Issue 4
Abstract
Aims: In the present study different extracts of seeds of Gmelina arborea were prepared and evaluated their membrane stabilizing and antioxidant effects. The Fatty acid composition was also estimated. Study Design: Fatty acid analysis by GCMS and evaluation of membrane stabilizing and antioxidant activity by hypotonic solution induced hemolysis and DPPH method respectively. Place and Duration of Study: Department of Pharmaceutical Chemistry, Dolphin PG Institute of Biomedical and Natural Sciences, Dehradun, India, between October 2013 to September 2014. Methodology: All extract were tested for presence of phytoconstituents i.e., alkaloid, carbohydrate, sterols, proteins, amino acids, saponin, and phenolic compounds in different extracts. Membrane Stabilizing effect was studied by hypotonic solution induced hemolysis of erythrocyte. Antioxidant activity was studied by DPPH method at a different concentration 50 μg/ml to 500 μg/ml. GCMS analysis was done for petroleum ether extract with the help of Perkin Elmer Clarus-500 model coupled with CLARUS-500 Mass spectrometer. Results: Phytochemical analysis showed the presence of alkaloid, carbohydrate, sterols, proteins, amino acids, saponin, and phenolic compounds in different extracts. Methanol extract was the richest extract for tested phytoconstituents. Different fatty acids were present in petroleum ether extract which was analyzed by GCMS. Maximum membrane stabilizing activity of seeds of Gmelina arborea showed in acetone (45.39±0.84) and methanol (44.12±0.72) extracts at a concentration of 2000 µg/ml in comparison to standard drug. From antioxidant studies, methanol extract showed maximum antioxidant activity (91.99±0.46) at a concentration of 500 µg/ml than other extract in comparison to standard drug ascorbic acid. Conclusion: From above studies it could be concluded that methanol and acetone extract showed maximum membrane stabilizing and antioxidant activities.
Authors and Affiliations
Deepak Kumar, Ashwani Sanghi, Raju Chandra, Shefali Arora, Ashwani Kumar Sharma
Keywords
Related Articles
- EP ID EP344332
- DOI 10.9734/BJPR/2015/16043
- Views 109
- Downloads 0
Phytochemical Identification in the Chloroform Fraction of Aqueous-Methanol Extract of Cnidoscolus aconitifolius Leaves
Aims: This study is designed to identify minor bioactive compounds present in 80% aqueous-methanol chloroform fraction of Cnidoscolus aconitifolius (CA) air-dried leaves using proton Nuclear Magnetic Resonance (1H NMR) s...
Determination of Vitamin B6, B9 and B12 in Halophytes by Solid Phase Extraction Followed by HPLC-UV
In this work, solid phase extraction (SPE) was proposed for simultaneous determination of trace levels of vitamin B6, B9 and B12. High performance liquid chromatography with UV detection (HPLC-UV) was used. The experimen...
Ethanol Extracts of Terminalia ivorensis (Chev A.) Stem Bark Attenuates the Positive, Negative and Cognitive Symptoms of Psychosis in Experimental Animal Models
Aims: This study was undertaken to evaluate the antipsychotic property of the ethanol extract of T. ivorensis (EETI) stem bark in mice Study Design: The study used experimental animal models predictive of human psych...
Analysis of Fenoterol and Ipratropium Transfer from Human Lung Tissue into Human Plasma Using a Dynamic Dialysis Model
Aims: The aim of the current study was to establish a simple and yet as much as possible physiologic approach for a simulation of the pulmonary absorption process to compare different inhaled drugs or drug formulations....
Perceptions of the Traditional Medical Practitioners of North-Western Nigeria on Malaria Treatment and the Potential Antiplasmodial Properties of Plumeria rubra Stem-Bark
Aims: The apparent lack of scientific proof of efficacies claimed by the traditional medical practitioners (TMPs) (locally known as Magori/’Yan-ganye, in Hausa language) of North-Western Nigeria with respect to malaria a...