Meristem culture for the elimination of potato virus S in plants grown in vitro
Journal Title: Biotecnología Vegetal - Year 2001, Vol 1, Issue 2
Abstract
The virus S of potato was detected in three varieties of scientific and productive interests during propagation in vitro of different genotypes. With the objective of obtaining plants free of virus, three sizes of meristems (0.3 to 0.2mm, 0.2 to 0.1mm, and 0.1 to 0.06mm) were studied. For the growth of the meristems a culture medium was used that contained Murashige and Skoog (1962) salts, 1 mg.l -1 thyamine, 100 mg.l -1 myoinositol, 1 mg.l - 1 gibberelic acid and 1.8 mg.l -1 gelling agent. The plants regenerated from meristems and after two transfers to multiplication culture medium were diagnosed through the ELISA technique. As a result 96.6% of cleaness was obtained when the range of meristem size was from 0.1 to 0.06 mm. The regeneration percentages of plants from the meristems decreased, as the range of size was smaller.
Authors and Affiliations
Leyanis García-Águila, Zoe Sarría, Tatiana Pichardo, Blanca Pérez
Keywords
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