Metagenomic Analysis: Microbial Diversity of Hot Spring Water Near Mumbai Region (India)
Journal Title: International Journal of Interdisciplinary and Multidisciplinary Studies (IJIMS) - Year 2017, Vol 4, Issue 3
Abstract
A large diversity of microorganisms are being harbored by natural environments and most of them have not yet been identified /or characterized. Extremophilic microorganisms are known to survive in diverse extreme conditions, such as high or low temperatures, high salinity etc.Thermophiles and hyper-thermophiles have the ability to survive in environments with very high temperature such as in hot springs. Sample was collected from hot spring near Nimbavali village, Ganeshpuri, Mumbai region, Maharashtra, India. The bacterial diversity as determined by sequencing the hypervariable region V3 of the 16S rRNA belonging to the microbial community of the Ganeshpuri (Nimbavali) hot spring is presented here. In the taxonomy classification of OTUs, only top ten OTUs were selected and rests were shown as others. Top ten OTUs at phylum level wereProteobacteria, Firmicutes,Bacteriodetes;class- Gammaprotobactera, Betaprotobacteria, Clostridia; Order- Clostridiales, Bacteriodales, Rhodothermales; family- Halobacteriaece,Comamondaceae; genusProtovella, Oscillospira;and species- Bifidobacteriumadolescentis. In all of the above significant percentage of OTUs were found to be unknown or not yet classified. These unknown microbes could serve as the source of novel products. Our data provide new information on hot spring bacteria and shed light on their abundance, diversity, distribution and coexisting organisms.
Authors and Affiliations
Deepak Mishra, Neeta Khanolkar
Keywords
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