METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF NEVIRAPINE FROM TABLETS BY RP-HPLC
Journal Title: International Journal of Pharmacy - Year 2011, Vol 1, Issue 1
Abstract
A reverse phase high performance liquid chromatography method has been developed for the estimation of nevirapine in tablets. The quantification was carried out on the symmetry C18 column, with a mobile phase consisting of acetonitrile and phosphate buffer in the ratio of 65:35 v/v. The mobile phase pumped at a rate of 0.8 mL/min and the detection was carried out at 283 nm. The linearity was found to be in the range of 20-60 μg/mL. The limit of detection and limit of quantitation was found to be 0.027μg/mL and 0.09μg/mL, respectively. The percentage recovery values were found to be in the range of 99.83-100.73%. Statistical analysis proves that the method was found to be simple, precise, accurate and reproducible, and can be used for the routine quality control of nevirapine in formulations.
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