Method development and validation for the simultaneous estimation of Atazanavir and Ritonavir in tablet dosage form by RP-HPLC
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 6
Abstract
The present investigation describes about a simple, economic, selective, accurate, precise reverse phase high performance liquid chromatographic method for the simultaneous estimation of Atazanavir and Ritonavir in pure and pharmaceutical dosage forms of Atazanavir and Ritonavir were well separated using a X-Tera C18 (100 x 4.6mm, 3.5m) and Mobile phase consisting of Buffer(pH-2.5): Acetonitrile (40:60) adjusted to pH- 2.5 at the flow rate 1.2 ml/min and the detection was carried out at 247nm with PDA detector. The Retention time for Atazanavir and Ritonavir were found to be 1.982 & 2.576 respectively. The developed method was validated for recovery, specificity, precision, accuracy, linearity according to ICH guidelines. The method was successfully applied to Metronidazole and Norfloxacin combination pharmaceutical dosage form.
Authors and Affiliations
Nuli Vasavi, Afroz Patan
Keywords
Related Articles
- EP ID EP33164
- DOI -
- Views 275
- Downloads 0
Amlodipine: the upcoming threat to Periodontist
Gingival hyperplasia is one of the side effects commonly caused by three major groups of drugs such as a) anticonvulsants b) antihypertensives – calcium channel blockers c) immunosuppressants. Previously among antihype...
DESIGN AND DEVELOPMENT OF AMLODIPINE BESYLATE FAST DISSOLVING TABLETS BY USING NATURAL SUPERDISINTEGRANTS
Amlodipine besylate is a long-acting calcium channel blocker used in the treatment of chronic stable angina, vasospastic angina and hypertension. Amlodipine besylate is a sparingly soluble orally administered drug and...
Evaluation of methanolic extract of Celtis timorensis for nootropic activity
The present study was undertaken to investigate the nootropic activity of leaves of methanolic extract of Celtis Timorensis. Three doses of extracts 100, 200 and 400 mg/kg and piracetam (50 mg/kg, i.p.) were administer...
Development and optimization of Diltiazem hydrochloride loaded microspheres by using different Eudragit polymers
The problems of frequent administration and variable low bioavailability (40-60%) after oral administration of conventional dosage forms of Diltiazem hydrochloride can be attenuated by designing it in the form of micro...
Flurbiprofen: β-cyclodextrin formulations: an improved method for fast dissolving tablet
The goal of the present investigation was to design and evaluated taste mask of fast dissolving tablet of Flurbiprofen, by addition of super-disintegrant method using various excipients ,Ac-di-sol, Avicel, Manitol and...