Method development and validation for the simultaneous estimation of Atazanavir and Ritonavir in tablet dosage form by RP-HPLC
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 6
Abstract
The present investigation describes about a simple, economic, selective, accurate, precise reverse phase high performance liquid chromatographic method for the simultaneous estimation of Atazanavir and Ritonavir in pure and pharmaceutical dosage forms of Atazanavir and Ritonavir were well separated using a X-Tera C18 (100 x 4.6mm, 3.5m) and Mobile phase consisting of Buffer(pH-2.5): Acetonitrile (40:60) adjusted to pH- 2.5 at the flow rate 1.2 ml/min and the detection was carried out at 247nm with PDA detector. The Retention time for Atazanavir and Ritonavir were found to be 1.982 & 2.576 respectively. The developed method was validated for recovery, specificity, precision, accuracy, linearity according to ICH guidelines. The method was successfully applied to Metronidazole and Norfloxacin combination pharmaceutical dosage form.
Authors and Affiliations
Nuli Vasavi, Afroz Patan
COMPARISION OF POTENCY OF ANTI BACTERIAL ACTIVITY AND ANTI INFLAMMATORY ACTIVITY OF 10 YEARS AND 100 YEARS OLD BARK EXTRACTS OF AZADIRACHTA INDICA
Azadirachta indica (Meliaceae) commonly known as neem contains many biologically active compounds including alkaloids, flavonoids, triterpenoids, phenolic compounds, Carotenoids, steroids and ketones, azadirachtin. Oil...
A validated RP HPLC method for simultaneous estimation of lamivudine and tenofovir disoproxil fumarate
A precise, simple, selective and accurate RP-HPLC method was developed and validated for rapid assay of Lamivudine and Tenofovir fumarate in pharmaceutical dosage form. Lamivudine and Tenofovir disoproxil fumarate are...
Effect of potato presence on the chemical and rheological properties of frying oil and physical properties of produced potato chips
In this study the effect of frying times and heating times on rheological and chemical properties of frying oil were studied. Results showed that rheological properties of frying oil did not affected by frying times an...
Development and validation of new RP-HPLC method for simultaneous estimation of drug Thiocolchicoside and Etodolac in tablet dosage form
A simple, economic, accurate and precise reverse phase high performance liquid chromatography method for analysis of Thiocolchicoside and Etodolac was developed and validated according to ICH guidelines. The quantifica...
One- pot synthesis of 2, 4, 5- trisubstituted imidazoles using ZrO2 catalyst
For the first time ZrO2 catalyst was used for the synthesis of 2, 4, 5- trisubstituted imidazoles. Tetragonal phase of ZrO2 is responsible for the good yields in conventional and microwave method. In microwave method b...