Method development and validation for the simultaneous estimation of Ofloxacin and Tinidazole in bulk and pharmaceutical dosage form by reverse phase HPLC method
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 6
Abstract
A new, simple, accurate, rapid, precise RP-HPLC method was developed for the simultaneous estimation of ofloxacin and tinidazole in bulk and pharmaceutical dosage form. A good chromatographic separation was achieved with Intersil ODS C18 (250×4.6mm.5µ) column and ammonium acetate buffer pH 4.0, acetonitrile, and tetrahydrofuran 60:30:10 was used as mobile phase at a flow rate of 1.0ml/min and the detection was carried out at a wavelength of 304 nm. The retention times was found to be 2.350 min for ofloxacin and 3.613 min for tinidazole. The linearity of the method was in the concentration range of 15-35µg/mL for ofloxacin and 45-105µg/mL for tinidazole. The developed method was validated for system suitability, specificity, precision, recovery and linearity according to ICH guidelines. The method was successfully applied for routine analysis for the determination of oflaxacin and tinidazole in bulk and dosage forms.
Authors and Affiliations
Y. Bhargav, K Haritha Pavani, S Amareswari
Keywords
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