METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC ANALYSIS OF BROMFENAC IN RABBIT PLASMA
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 8
Abstract
A simple, precise, rapid, and accurate reverse phase high performance liquid chromatography method was developed for the estimation of Bromfenac in Rabbit plasma by using Paracetamol as IS. The protein precipitation technique was used for the extraction of analyte from rabbit plasma. The chromatographic separation was performed on Agilent, Zorbas SB C18 (4.6mm×250mm×5μ) column with PDA detection at 267nm. The mobile phase comprising of mixture of methanol and buffer 0.1% ortho phosphoric acid (pH 2.6) in the ratio of 30:70v/v, at the flow rate 0.8ml/min. The analytical method described here is valid for the estimation of Bromfenac in Rabbit plasma over a range of 50μg/ml-150μg/ml. The retention times of Bromfenac and Paracetamol were found to be 3.781 and 2.470mins respectively. The correlation coefficient for Paracetamol and Bromfenac were 0.998 and 0.999 respectively. The LOD and LOQ values for Bromfenac were found to be 0.13μg/ml and 0.40μg/ml respectively. %RSD of Bromfenac and Paracetamol was obtained to be 0.41and 0.28 respectively. This method is time efficient and samples are easy to monitoring Bromfenac in plasma.
Authors and Affiliations
Vidya Kamdar , Uma Maheshwara Rao , Vattikuti , Ajitha Azhakesan
Keywords
Related Articles
- EP ID EP112008
- DOI -
- Views 110
- Downloads 0
METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF TRIFLUOPERAZINE AND ISOPROPAMIDE IN TABLET DOSAGE FORM BY RP-HPLC
A simple, precise, rapid, specific and accurate reverse phase high performance liquid chromatography method was developed for simultaneous estimation of Trifluoperzine and Isopropamide in pharmaceutical dosage form. Chro...
ASSAY METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF PAROXETINE AND CLONAZEPAM BY RP- HPLC
A simple, precise, rapid, specific and accurate reverse phase high performance liquid chromatography method was developed for simultaneous estimation of Paroxetine and Clonazepam in pharmaceutical dosage form. Chromatogr...
A REVIEW ON CURRENT TRENDS AND ADVANCEMENTS IN BIOANALYTICAL METHODS
This paper reviews the recent developments in bio-analysis sample preparation techniques and chromatographic techniques give an update on basic principles and a comparative discussion on the benefits and limitation of ev...
DEVELOPMENT AND VALIDATION OF UV- SPECTROSCOPIC METHOD FOR AMLODIPINE BESYLATE, OLMESARTAN MEDOXOMIL, HYDROCHLOROTHIAZIDE IN PURE AND TABLET DOSAGE FORM
The objective of the present study to develop and validation of Amlodipine Besylate, Olmesartan Medoxomil, Hydrochlorothiazide in bulk and in combined tablet formulation by UV Spectrophotometric method. Blood pressure lo...
ISOLATION, PURIFICATION AND BIOPHYSICAL CHARACTERIZATION OF BASIC 7S GLOBULIN FROM COCOS NUCIFERA
Elucidation of structure-function relationships of seed storage proteins is a prerequisite for developing theoretically new food and/or food materials based on seed storage proteins. Furthermore, recent findings suggest...