METHOD DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF DARUNAVIR IN BULK AND ITS PHARMACEUTICAL DOSAGE FORM
Journal Title: Indo American Journal of Pharmaceutical Sciences - Year 2018, Vol 5, Issue 4
Abstract
Two simple, precise and economical UV Spectrophotometric methods have been developed for the estimation of Darunavir ethanolate (DRV) in bulk and its pharmaceutical dosage form. Two methods were developed based on measurement of absorption at maximum wavelengths, for Method Ⅰ 272.1 nm and Method ⅠⅠ 272.4 nm. Linearity for detector response was observed in the concentration range of 2-10 μg/ml for the both methods. The developed methods were validated with respect to linearity, accuracy (recovery), precision and specificity. The accuracy of the methods was assessed by recovery studies and was found to be 98.3% and 99% for Method Ⅰ and Method ⅠⅠ respectively. The results were validated statistically as per ICH Q2 R1 guidelines and were found to be satisfactory. The proposed methods were successfully applied for the determination of DRV in pharmaceutical dosage form. Key words: Darunavir, UV Spectrophotometry, Absorbance maxima methods and Validation.
Authors and Affiliations
M. M. Eswarudu*, P. Srinivasa Babu, P. Siva Krishna, G. Sneha Latha, N. Anusha, M. S. K. Mounika and N. Punna Reddy. M. M. Eswarudu
Keywords
Related Articles
- EP ID EP278363
- DOI -
- Views 290
- Downloads 0
HERBAL EXTRACT ENCAPSULATED IN CHITOSAN NANOPARTICLE: A NOVEL STRATEGY FOR THE TREATMENT OF UROLITHIASIS
In current study the inhibitory potential of Tridax procumbens leaf extract loading chitosan nanoparticle against calcium oxalate crystallization under in vitro condition. Kidney stone formation was studied using three a...
CHRONIC DIARREHA: FREQUENCY OF CELIAC DISEASE IN CHILDERNS AT TEATIARY CARE HOSPITAL
Introduction: Celiac disease (CD) is an autoimmune disease of chronic origin affecting children as well adults and characterized by an unusual response towards gluten present in diet particularly in subjects who are gene...
RP-HPLC PDA METHOD FOR ESTIMATION OF ZANAMIVIR IN API AND PHARMACEUTICAL FORMULATION
A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Zanamivir in its pure form as well as in tablet dosage form. Chromatography was carried out on Sunf...
A NEW RP-HPLC METHOD FOR THE SIMULATANEOUS ESTIMATION OF NEBIVOLOL AND VALSARTAN IN TABLETS
A simple RP-HPLC method was developed and validated for the simultaneous estimation of Valsartan and Nebivolol in tablets. The Valsartan and Nebivolol were analyzed by RP-HPLC using Hypersil BDS column (150 cm X 4.6 mm,...
FORMULATION AND EVALUATION OF CHITOSAN NANOPARTICLE LOADED WITH PHYLLANTHIN
Objective: The aim of the work was to formulate and evaluate chitosan nanoparticle loaded with Phyllanthin. Methods: Chitosan nanoparticle loaded with phyllanthin were prepared by ionic chelation method using chitosan so...