METHOD DEVELOPMENT FOR QUANTIFICATION OF LORATIDINE AND ALVERINE CITRATE BY VISIBLE SPECTROPHOTOMETRY

Abstract

The present work describes on two colorimetric methods based on charge transfer complex reaction of loratidine (LRT) and alverine citrate (ALV) with chloranilic acid in chloroform. The methods were developed on Perkin Elmer LAMBDA 25 UV –VIS spectrophotometer with 1cm quartz cells. The methods were optimised to achieve maximum colour intensity and validated for reliability. The coloured complexes showed maximum absorbance at 538 nm for LRT and 540 nm for ALV. The absorbances were found to increase linearly with increase in concentration which was emphasised by the calculated regression coefficients (0.9998-0.9999). Linearity of standard plot was in the order of 100-700 and 80-560µg/ml for LRT and ALV, respectively. The molar absorptivity, sandells sensitivity, LOD, LOQ and other validation parameters have been assessed extensively and all the parameters seem to comply with the acceptance criteria. The proposed methods were proved to be more accurate, simple, precise and rapid by statistical validation, recovery studies and could be appropriate to employ in regular laboratory analysis.

Authors and Affiliations

Vijayalakshmi R. , Naga Sri Ramya Y, Dhanaraju MD

Keywords

Related Articles

EFFECTS OF CURCUMIN ANALOGUE, 2, 6-BIS (2, 5-DIMETHOXYBENZYLIDENE) CYCLOHEXANONE (BDMC33) ON THE ACTIVITIES OF DRUG-METABOLIZING ENZYMES IN CULTURED CACO-2 CELL MODEL

Poor systemic delivery of curcumin outside the gut due to its rapid metabolism has severely limited its application to many chronic diseases. Previously, our research group synthesized curcumin analogues 2, 6-bis (2, 5-d...

DEVELOPMENT AND IN VIVO EVALUATION LOVASTATIN BY SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEM

In the present investigation, self-nanoemulsifying drug delivery system (SNEDDS), of Lovastatin is being formulated to increase the solubility and bioavailability. The optimized Lovastatin SNEDDS formulation (F8) has a c...

QUALITY CONTROL OF A MARINE ORIGIN BASED HERBO-MINERAL UNANI FORMULATION

Kushta Marjan (KM) is one well-known formulation prepared from coral used by Unani physicians for effective treatment of a various ailments. Coral is the calcareous skeleton of the minute marine organism and belongs of p...

GAS CHROMATOGRAPHY METHOD OF CLEANING VALIDATION PROCESS FOR 2-PROPANOL RESIDUE DETERMINATION IN PHARMACEUTICAL MANUFACTURING EQUIPMENT

Cleaning validation is an integral operation of good manufacturing practice in pharmaceutical industry. The aim of this study was to validate simple analytical method for detection of 2-propanol residue in equipment, whi...

Antipsychotic Prescribing Pattern in Elderly Patients with Psychiatric Disorders

The elderly people are more prone to develop psychiatric disorders due to a number of factors like changes in the brain, other illnesses etc. They represent the most vulnerable group as they are most sensitive to the eff...

Download PDF file
  • EP ID EP625176
  • DOI 10.25004/IJPSDR.2016.080307
  • Views 90
  • Downloads 0

How To Cite

Vijayalakshmi R. , Naga Sri Ramya Y, Dhanaraju MD (2016). METHOD DEVELOPMENT FOR QUANTIFICATION OF LORATIDINE AND ALVERINE CITRATE BY VISIBLE SPECTROPHOTOMETRY. International Journal of Pharmaceutical Sciences and Drug Research, 8(3), 166-169. https://europub.co.uk/articles/-A-625176